Analytical Data
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基因名
PEDF
- Application
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别名
SERPINF1;PEDF;Pigment epithelium-derived factor
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P36955
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表达区间
20-418aa
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氨基酸序列
QNPASPPEEGSPDPDSTGALVEEEDPFFKVPVNKLAAAVSNFGYDLYRVR SSTSPTTNVLLSPLSVATALSALSLGAEQRTESIIHRALYYDLISSPDIH GTYKELLDTVTAPQKNLKSASRIVFEKKLRIKSSFVAPLEKSYGTRPRVL TGNPRLDLQEINNWVQAQMKGKLARSTKEIPDEISILLLGVAHFKGQWVT KFDSRKTSLEDFYLDEERTVRVPMMSDPKAVLRYGLDSDLSCKIAQLPLT GSMSIIFFLPLKVTQNLTLIEESLTSEFIHDIDRELKTVQAVLTVPKLKL SYEGEVTKSLQEMKLQSLFDSPDFSKITGKPIKLTQVEHRAGFEWNEDGA GTTPSPGLQPAHLTFPLDYHLNQPFIFVLRDTDTGALLFIGKILDPRGP
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分子量
44 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Pigment epithelium-derived factor (PEDF) is a unique serpin that has garnered significant attention in medical research due to its diverse biological roles, particularly in neuroprotection, anti-angiogenesis, and cancer inhibition. Initially discovered as a retinal pigment epithelium-secreted protein, PEDF plays a crucial role in the maintenance of retinal health and has been implicated in various ocular diseases such as diabetic retinopathy and age-related macular degeneration. Beyond ocular applications, PEDF is recognized for its neuroprotective properties, showing promise in protecting neuronal cells from degeneration due to oxidative stress and apoptosis. Research has also highlighted its anti-angiogenic effects, making it a potential therapeutic candidate for inhibiting pathological blood vessel formation in tumors and other diseases. Furthermore, studies investigating the recombinant production of PEDF protein have aimed to explore its functional mechanisms in numerous biological pathways, enabling better understanding and potential applications in regenerative medicine and cancer therapy. The development of effective recombinant PEDF has opened new avenues for therapeutic intervention, making it an attractive focus of research in pharmacology and biotechnology.
