Analytical Data
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Gene name
gltA
- Application
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Alternative Names
gltA；Glutamate synthase [NADPH] large chain
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P56062
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Expression Region
1-426aa
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AA Sequence
MSVTLVNNENNERYEFETIESTRGPKAVDFSKLFETTGFFSYDPGYSSTAGCQSKISYVNGKKGELYYRGHRIEDLVAKYKYVDVCKLLLTGELPKNQDESLEFELELRHRSFVHESLLNMFSAFPSNAHPMAKLSSGVSILSTLYSTHQNMHTEEDYQTMARRIVAKIPTLAAICYRNEVGAPIIYPDIARSYVENILFMLRGYPYSRLKHTTQGEVEITPLEVEAFDKILTLHADHSQNASSTTVRNVASTGVHPYAAISAGISALWGHLHGGANEKVLLQLEEIGDVKNVDKYIARVKDKNDNFKLMGFGHRVYKSYDPRAKILKGLKDELHQKGVKMDERLSEIAAKVEEIALKDEYFIERNLYPNVDFYSGTILRALKIPVRFFTPVFVIGRTVGWCAQLLEHVKSPQARITRPRQVYVGD
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Molecular Weight
55.9kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
GltA, or citrate synthase, is a key enzyme in the citric acid cycle, crucial for energy metabolism in various organisms, including bacteria, plants, and animals. Research on GltA recombinant protein has garnered significant interest due to its pivotal role in cellular respiration and metabolic pathways. Understanding the structure and function of GltA provides insights into its mechanism of action and regulation, making it a target for pharmaceutical interventions in metabolic disorders and infectious diseases. Furthermore, recombinant GltA can be utilized in biotechnological applications, such as the production of biochemicals and biofuels. The cloning, expression, and purification of GltA in various expression systems enable researchers to study its enzymatic properties, interactions with substrates and inhibitors, and its role in metabolic engineering. Additionally, investigating GltA's evolutionary adaptations can shed light on the adaptation of different species to their environments. Overall, GltA's significance in metabolism and its potential applications in medicine and biotechnology make it a compelling subject for research, driving efforts to elucidate its intricate biology and explore new avenues for its application.
