Analytical Data
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基因名
MBD5
- Application
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别名
9430004D19Rik; AA536666; AI426407; C030040A15Rik; FLJ11113; FLJ30517; KIAA1461 ; MBD5; MBD5_HUMAN; methyl CpG binding domain protein 5; Methyl CpG binding protein MBD5 ; Methyl-CpG-binding domain protein 5; Methyl-CpG-binding protein MBD5; RP23 167N13.1
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9P267
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表达区间
1-229aa
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氨基酸序列
MFPPTANMLLPTGEGQSGRAALRDKLMSQQKDALRKRKQPPTTVLSLLRQSQMDSSAVPKPGPDLLRKQGQGSFPISSMSQLLQSMSCQSSHLSSNSTPGCGASNTALPCSANQLHFTDPSMNSSVLQNIPLRGEAVHCHNANTNFVHSNSPVPNHHLAGLINQIQASGNCGMLSQSGMALGNSLHPNPPQSRISTSSTPVIPNSIVSSYNQTSSEAGMVLLEKSTQRY
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分子量
50.93 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
MBD5 (Methyl-CpG Binding Domain Protein 5) is a member of the MBD family of proteins, which are known for their roles in recognizing and binding to methylated DNA, thus playing a crucial role in gene regulation and epigenetic modifications. Research on MBD5 has gained prominence due to its association with neurodevelopmental disorders, particularly the 2q23.1 deletion syndrome, which implicates MBD5 as a significant player in cognitive functions and developmental processes. Mutations or disruptions in MBD5 have been linked to various phenotypic manifestations, including intellectual disabilities, speech delays, and other neurobehavioral issues. Given the importance of MBD5 in the regulation of gene expression and its potential implications in disease, researchers are focused on characterizing the structure and function of MBD5 through recombinant protein studies. These investigations aim to elucidate the protein's binding affinities, interaction partners, and downstream effects on gene expression. By analyzing the recombinant MBD5 protein, scientists hope to uncover insights into its biological mechanisms and how dysregulation contributes to pathologies, thereby paving the way for potential therapeutic strategies targeting MBD5-related disorders. Furthermore, understanding MBD5's role in methylation dynamics can enhance our knowledge of epigenetic regulation in broader contexts, potentially influencing fields ranging from developmental biology to cancer research.
