Analytical Data
-
Gene name
MBIP
- Application
-
Alternative Names
MAP3K12 binding inhibitory protein 1; MAP3K12-binding inhibitory protein 1; MAPK upstream kinase binding inhibitory protein; MAPK upstream kinase-binding inhibitory protein; Mbip; MBIP1_HUMAN
-
Species
Human
-
Source
E. coli
-
Tag
GST-tag at N-terminal
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
Q9NS73
-
Expression Region
1-344aa
-
AA Sequence
MAAATEHNRPSSGDRNLERRCSPNLSREVLYEIFRSLHTLVGQLDLRDDVVKITIDWNKLQSLSAFQPALLFSALEQHILYLQPFLAKLQSPIKEENTTAVEETGRTEMGNKNEVNDKFSIGDLQEEEKHKESDLRDVKKTQIHFDPEVVQIKAGKAEIDRRISAFIERKQAEINENNVREFCNVIDCNQENSCARTDAIFTPYPGFKSHVKVSRVVYTYGPQTRPEGIPGSGHKPNSMLRDCGNQAVEERLQNIEAHLRLQTGGPVPRDIYQRIKKLEDKILELEGISPEYFQSVSFSGKRRKVQPPQQNYSLAELDEKISALKQALLRKSREAESMATHHLP
-
Molecular Weight
63.58 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
MBIP (MCL1 Binding Protein) is an essential scaffolding protein that plays a crucial role in the regulation of cell survival and apoptosis by interacting with MCL1, a member of the BCL-2 family of proteins. Dysregulation of MCL1 has been implicated in various cancers, making the understanding of MBIP's function and its interactions particularly significant for therapeutic development. Research has shown that MBIP not only stabilizes MCL1 but also modulates its activity by influencing its localization and degradation. As cancer therapies increasingly target the apoptotic pathways, elucidating the structural and functional aspects of MBIP can provide insights into potential drug development. Furthermore, understanding the reconstitution of MBIP as a recombinant protein can facilitate in vitro studies, allowing for detailed exploration of its biochemical properties and interactions with MCL1 and other cellular proteins. This research is pivotal for uncovering the molecular mechanisms underlying cancer progression and resistance, ultimately paving the way for innovative treatment strategies aimed at restoring apoptotic functions in cancer cells.
