Analytical Data
-
Gene name
PCMTD1
- Application
-
Alternative Names
PCMTD1;Protein-L-isoaspartate O-methyltransferase domain-containing Protein 1
-
Species
Human
-
Source
E. coli
-
Tag
His tag N-Terminus
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
Q96MG8
-
Expression Region
1-220aa
-
AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSMGGAVSAGEDNDDLIDNLKEAQYIRTERVEQAFRAIDRGDYYLEGYR DNAYKDLAWKHGNIHLSAPCIYSEVMEALKLQPGLSFLNLGSGTGYLSTM VGLILGPFGINHGIELHSDVVEYAKEKLESFIKNSDSFDKFEFCEPAFVV GNCLQIASDSHQYDRIYCGAGVQKDHENYMKILLKVGGILVMPIEDQLTQ IMRTGQNTWESKNILAVSFAPLV
-
Molecular Weight
27 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PCMTD1 (Protein-L-isoaspartate O-methyltransferase domain-containing protein 1) is a member of the methyltransferase family, implicated in the post-translational modification of proteins. Its primary role is to repair damaged proteins by methylating L-isoaspartate residues, which accumulate due to non-enzymatic reactions that occur over time, particularly under stress conditions. The presence of these non-canonical amino acids can adversely affect protein function, stability, and cellular homeostasis, leading to various diseases, including neurodegenerative disorders and age-related pathologies. Recent studies have highlighted the important function of PCMTD1 in cellular protection mechanisms, demonstrating its potential as both a biomarker for stress-related conditions and a therapeutic target. The exploration of PCMTD1 involves understanding its enzymatic activity, structural features, and regulatory pathways. Additionally, ongoing research aims to delineate its interactions with other proteins and cellular components, which can uncover novel insights into its role in maintaining protein integrity and cellular health. Understanding the molecular mechanisms and biological significance of PCMTD1 is crucial for developing strategies to mitigate protein misfolding and aggregation, thus advancing therapeutic approaches in age-associated diseases and neurodegeneration.
