Analytical Data
-
Gene name
PCK1
- Application
-
Alternative Names
PCK1;PEPCK1;Phosphoenolpyruvate carboxykinase. cytosolic [GTP]
-
Species
Human
-
Source
E. coli
-
Tag
His tag N-Terminus
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
P35558
-
Expression Region
2-622aa
-
AA Sequence
PPQLQNGLNLSAKVVQGSLDSLPQAVREFLENNAELCQPDHIHICDGSEE ENGRLLGQMEEEGILRRLKKYDNCWLALTDPRDVARIESKTVIVTQEQRD TVPIPKTGLSQLGRWMSEEDFEKAFNARFPGCMKGRTMYVIPFSMGPLGS PLSKIGIELTDSPYVVASMRIMTRMGTPVLEAVGDGEFVKCLHSVGCPLP LQKPLVNNWPCNPELTLIAHLPDRREIISFGSGYGGNSLLGKKCFALRMA SRLAKEEGWLAEHMLILGITNPEGEKKYLAAAFPSACGKTNLAMMNPSLP GWKVECVGDDIAWMKFDAQGHLRAINPENGFFGVAPGTSVKTNPNAIKTI QKNTIFTNVAETSDGGVYWEGIDEPLASGVTITSWKNKEWSSEDGEPCAH PNSRFCTPASQCPIIDAAWESPEGVPIEGIIFGGRRPAGVPLVYEALSWQ HGVFVGAAMRSEATAAAEHKGKIIMHDPFAMRPFFGYNFGKYLAHWLSMA QHPAAKLPKIFHVNWFRKDKEGKFLWPGFGENSRVLEWMFNRIDGKASTK LTPIGYIPKEDALNLKGLGHINMMELFSISKEFWEKEVEDIEKYLEDQVN ADLPCEIEREILALKQRISQM
-
Molecular Weight
71 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PCK1, or phosphoenolpyruvate carboxykinase 1, is a crucial enzyme that plays a significant role in gluconeogenesis, the metabolic pathway responsible for glucose production from non-carbohydrate sources. It catalyzes the conversion of oxaloacetate to phosphoenolpyruvate, a key step in the synthesis of glucose in the liver and kidneys. Aberrant expression or regulation of PCK1 is implicated in various metabolic disorders, including diabetes and obesity, making it a potential target for therapeutic interventions. Additionally, the understanding of PCK1's regulatory mechanisms and its interaction with other metabolic pathways is of great interest in biochemistry and molecular biology. The recombinant expression of PCK1 in suitable host systems allows for the production of large quantities of the enzyme for detailed biochemical characterization, aiding in the elucidation of its structure-function relationship and its role in metabolic regulation. Furthermore, studying PCK1 can provide insights into the development of targeted treatments for metabolic diseases, as well as the exploration of its potential applications in biotechnological processes aimed at improving energy metabolism in various organisms. This heightened focus on PCK1 underscores its importance not only in basic research but also in the development of novel therapeutic strategies.
