Analytical Data
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Gene name
PTPRF
- Application
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Alternative Names
PTPRF；LAR；Receptor-type tyrosine-Protein phosphatase F
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P10586
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Expression Region
1275-1897aa
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AA Sequence
ILIVIAILLFKRKRTHSPSSKDEQSIGLKDSLLAHSSDPVEMRRLNYQTP GMRDHPPIPITDLADNIERLKANDGLKFSQEYESIDPGQQFTWENSNLEV NKPKNRYANVIAYDHSRVILTSIDGVPGSDYINANYIDGYRKQNAYIATQ GPLPETMGDFWRMVWEQRTATVVMMTRLEEKSRVKCDQYWPARGTETCGL IQVTLLDTVELATYTVRTFALHKSGSSEKRELRQFQFMAWPDHGVPEYPT PILAFLRRVKACNPLDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKTV DIYGHVTCMRSQRNYMVQTEDQYVFIHEALLEAATCGHTEVPARNLYAHI QKLGQVPPGESVTAMELEFKLLASSKAHTSRFISANLPCNKFKNRLVNIM PYELTRVCLQPIRGVEGSDYINASFLDGYRQQKAYIATQGPLAESTEDFW RMLWEHNSTIIVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNM PQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHK TKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTVKTL RTQRPAMVQTEDQYQLCYRAALE
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Molecular Weight
93 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PTPRF (Protein Tyrosine Phosphatase Receptor Type F) is a member of the protein tyrosine phosphatase (PTP) family, which plays a critical role in cellular signaling by removing phosphate groups from tyrosine residues on proteins. This function is essential for the regulation of various cellular processes, including growth, differentiation, and immune response. Dysregulation of PTPs has been implicated in numerous diseases, including cancer and autoimmune disorders. Due to its role in these pathological conditions, PTPRF has garnered attention as a potential therapeutic target. Researchers are increasingly interested in producing recombinant PTPRF proteins to study their structure, function, and interactions with other cellular components. The generation of recombinant PTPRF not only allows for the elucidation of its biochemical properties but also facilitates high-throughput screening of potential inhibitors or activators that could be used in clinical applications. Recent advances in recombinant DNA technology and protein expression systems have enabled more efficient production of this protein in sufficient quantities for various biochemical assays. Understanding the functional mechanisms of PTPRF through recombinant studies can provide insights into its role in health and disease, paving the way for novel approaches in disease treatment and drug development. As such, ongoing research efforts aim to unravel the complexities of PTPRF signaling pathways and their implications in human health, making it a focal point in molecular biology and therapeutic innovation.
