Analytical Data
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Gene name
MAD2L2
- Application
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Alternative Names
Homolog of REV7 S cerevisiae; hREV7; MAD2 (mitotic arrest deficient yeast. homolog) like 2; MAD2 homolog; MAD2 like 2; MAD2 mitotic arrest deficient like 2; MAD2-like protein 2; MAD2B ; Mad2l2; MD2L2_HUMAN; Mitotic Arrest Deficient 2 L2; Mitotic arrest deficient 2-like protein 2; Mitotic arrest deficient homolog like 2; Mitotic arrest deficient like 2 (yeast) ; Mitotic arrest deficient yeast homolog; Mitotic spindle assembly checkpoint protein MAD2B
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9UI95
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Expression Region
1-211aa
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AA Sequence
MTTLTRQDLN FGQVVADVLC EFLEVAVHLI LYVREVYPVG IFQKRKKYNV PVQMSCHPEL NQYIQDTLHC VKPLLEKNDV EKVVVVILDK EHRPVEKFVF EITQPPLLSI SSDSLLSHVE QLLRAFILKI SVCDAVLDHN PPGCTFTVLV HTREAATRNM EKIQVIKDFP WILADEQDVH MHDPRLIPLK TMTSDILKMQ LYVEERAHKG S
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Molecular Weight
24.3 KDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
MAD2L2, also known as MAD2B, is a member of the MAD2 family of proteins that plays a critical role in the regulation of the spindle assembly checkpoint (SAC) during cell division. This checkpoint ensures proper chromosome segregation and prevents aneuploidy, which is a hallmark of many cancers. Research has shown that MAD2L2 is involved in stabilizing the mitotic spindle and can interact with various proteins associated with the mitotic machinery, enhancing its importance in maintaining genomic stability. Recent studies have indicated that MAD2L2 can compensate for the loss of MAD2, the traditional SAC component, suggesting its potential role in alternative pathways to ensure fidelity during mitosis. Understanding the structural and functional properties of MAD2L2 could provide insights into its mechanisms of action and its potential implications in cancer biology. Developing recombinant MAD2L2 protein for biochemical assays and structural studies is vital to elucidating its role in cellular processes and understanding how its dysfunction may contribute to tumorigenesis. The investigation into the dynamics of MAD2L2 not only enriches our knowledge of the SAC but also opens new avenues for therapeutic interventions targeting the anomalies associated with cell division in cancer cells.
