Analytical Data
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基因名
NusA
- Application
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别名
DMC1;DMC1H;LIM15;Meiotic recombination Protein DMC1/LIM15 homolog
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种属
E.coli
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P0AFF6
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表达区间
1-495aa
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氨基酸序列
MNKEILAVVE AVSNEKALPR EKIFEALESA LATATKKKYE QEIDVRVQID RKSGDFDTFR RWLVVDEVTQ PTKEITLEAA RYEDESLNLG DYVEDQIESV TFDRITTQTA KQVIVQKVRE AERAMVVDQF REHEGEIITG VVKKVNRDNI SLDLGNNAEA VILREDMLPR ENFRPGDRVR GVLYSVRPEA RGAQLFVTRS KPEMLIELFR IEVPEIGEEV IEIKAAARDP GSRAKIAVKT NDKRIDPVGA CVGMRGARVQ AVSTELGGER IDIVLWDDNP AQFVINAMAP ADVASIVVDE DKHTMDIAVE AGNLAQAIGR NGQNVRLASQ LSGWELNVMT VDDLQAKHQA EAHAAIDTFT KYLDIDEDFA TVLVEEGFST LEELAYVPMK ELLEIEGLDE PTVEALRERA KNALATIAQA QEESLGDNKP ADDLLNLEGV DRDLAFKLAA RGVCTLEDLA EQGIDDLADI EGLTDEKAGA LIMAARNICW FGDEA
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
NusA is a crucial regulatory protein in Escherichia coli that plays a pivotal role in transcriptional regulation. It is known to enhance the elongation phase of RNA polymerase transcription and also acts as a transcription anti-terminator, allowing RNA polymerase to continue synthesizing RNA even in the presence of terminator sequences. The study of NusA has garnered significant interest due to its involvement in the regulation of various gene expressions and its potential role in bacterial survival mechanisms. Furthermore, NusA is implicated in the response to environmental stresses, making it an important target for understanding bacterial adaptability. Given its multifunctional properties, the recombinant production of NusA has become a subject of considerable research, facilitating the exploration of its molecular mechanisms and interactions with other transcription factors. By generating NusA as a recombinant protein, researchers can conduct in-depth biochemical and structural studies to illuminate its role in transcription regulation. This work has broader implications, potentially aiding the development of novel antimicrobial strategies that target transcriptional processes in bacteria. Through the understanding of NusA's function and interactions, scientists aim to elucidate the complexities of bacterial transcription regulation, providing insights that could inform therapeutic approaches in combating bacterial infections.
