Analytical Data
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Gene name
KPNa3
- Application
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Alternative Names
KPNa3；QIP2；Importin subunit alpha-4
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O00505
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Expression Region
2-521aa
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AA Sequence
AENPSLENH RIKSFKNKGR DVETMRRHRN EVTVELRKNK RDEHLLKKRN VPQEESLEDS DVDADFKAQN VTLEAILQNA TSDNPVVQLS AVQAARKLLS SDRNPPIDDL IKSGILPILV KCLERDDNPS LQFEAAWALT NIASGTSAQT QAVVQSNAVP LFLRLLRSPH QNVCEQAVWA LGNIIGDGPQ CRDYVISLGV VKPLLSFISP SIPITFLRNV TWVIVNLCRN KDPPPPMETV QEILPALCVL IYHTDINILV DTVWALSYLT DGGNEQIQMV IDSGVVPFLV PLLSHQEVKV QTAALRAVGN IVTGTDEQTQ VVLNCDVLSH FPNLLSHPKE KINKEAVWFL SNITAGNQQQ VQAVIDAGLI PMIIHQLAKG DFGTQKEAAW AISNLTISGR KDQVEYLVQQ NVIPPFCNLL SVKDSQVVQV VLDGLKNILI MAGDEASTIA EIIEECGGLE KIEVLQQHEN EDIYKLAFEI IDQYFSGDDI DEDPCLIPEA TQGGTYNFDP TANLQTKEFN F
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Molecular Weight
57.8 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
KPNa3, a member of the importin alpha family, plays a crucial role in nucleocytoplasmic transport, specifically in the import of proteins containing nuclear localization signals (NLS) into the nucleus. Its significance has drawn considerable attention in the context of various biological processes, including cell cycle regulation, gene expression, and signal transduction. Dysregulation of KPNa3 has been implicated in several diseases, including cancer, where altered nucleocytoplasmic transport can lead to aberrant localization of oncogenes and tumor suppressors. Research on KPNa3 recombinant proteins aims to elucidate its structure-function relationship, which can provide insights into its interaction with NLS-bearing proteins and other nuclear transport factors. Additionally, the development of KPNa3-based tools and assays can facilitate the discovery of novel therapeutic targets that modulate nuclear transport pathways. Understanding KPNa3's mechanisms at the molecular level is vital for both basic biological research and potential clinical applications, as it offers a promising avenue for targeting diseases associated with nuclear transport dysregulation.
