Analytical Data
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Gene name
cheA
- Application
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Alternative Names
cheA；POLDS；DNA polymerase delta subunit 4
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P07363
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Expression Region
1-654aa
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AA Sequence
MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIFRAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQLDAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPRRIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEADQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSNESTSIRVAVEKVDQLINLVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFSRYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKRLAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEVAMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQKMGGHVEIQSKQGTGTTIRILLPLTLAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWKVFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAATILGDGSVALIVDVSALQAINREQRMANTAA
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Molecular Weight
78.8 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
CheA is a crucial histidine kinase that plays a significant role in the chemotaxis signaling pathway in various bacteria, including Escherichia coli. As part of the two-component regulatory system, CheA is responsible for sensing environmental changes and transmitting signals that modulate the motility and behavioral responses of bacteria. The CheA protein undergoes autophosphorylation, which leads to the activation of downstream effectors, including CheY, which in turn influences flagellar rotation and, consequently, chemotactic behavior. Understanding the structure and function of CheA, particularly through the study of recombinant forms, is vital to elucidate the mechanisms underlying bacterial adaptation to their surroundings. Researchers employ recombinant DNA technology to produce CheA in a purified form, enabling detailed biochemical and structural analyses. These studies can reveal insights into the protein's intricate domain architecture, regulatory mechanisms, and interactions with other components of the chemotaxis signaling cascade. Additionally, exploring the dynamics of CheA can provide broader implications for microbial behavior, potential antibiotic targets, and biotechnological applications. In summary, recombinant CheA serves as a key model for understanding bacteria's sensory mechanisms and their adaptive strategies in fluctuating environments.
