Analytical Data
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Gene name
Cyp3a16
- Application
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Alternative Names
Cyp3a16；Cyp3a-16；Cytochrome P450 3A16
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q64481
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Expression Region
1-504aa
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AA Sequence
MNLFSALSLDTLVLLAIILVLLYRYGTCTHGLFKKQGIPGPKPLPFLGTVLNYYKGLWKFDMECYEKYGKTWGLFDGQIPLFVITDPETIKNVLVKECFSVFTNRQDFFPVGIMSKSISLAKDEEWKRYRALLSPTFTSGNLKEMFPVIEQYGDILVKYLRQEAEKGKPVAVKDVLGAYSMDVIISTTFGVNIDSLNNPEDPFVENAKKVLRFDYFDPLSLSVALFPFLTPIYEMLNICMFPKDSIEFFKKFVDRMTENRLDSKQKHRVDFIYLMMEAYNKSKDKDSHKALSEIEITAQSIIFIFAGYETTSSILSFTVYSLATHPDIQKKLQEEIDEALPNKAPPTYDTVMAMEYLDMVLNETLRLYPITNRLQRVCKKDVEINGIYIPKGSTVIIPSYVLHHDPQHWPEPEEFQPERFSKENKGSIDPYVYLPFGNGPRNCIGMRFALMNMKLALIKVLQNFSFQPCKETQIPLKLSRELLLQPVKPIVLKVVPRDAVITGA
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Molecular Weight
57.8 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Cytochrome P450 enzymes, particularly the Cyp3a family, play a critical role in the metabolism of a variety of xenobiotics and endogenous compounds. Among these, Cyp3a16 is a member predominantly expressed in the liver of certain species, where it participates in the biotransformation of drugs and toxins. Research on Cyp3a16 is significant due to its potential implications in pharmacogenomics, drug-drug interactions, and metabolic syndrome. Understanding the function and substrate specificity of Cyp3a16 through recombinant protein studies can provide insights into its role in therapeutic drug metabolism and adverse drug reactions. Furthermore, the analysis of this enzyme in different genetic backgrounds can shed light on variations in drug metabolism among individuals. Advancements in recombinant protein technology allow for the production of active Cyp3a16, facilitating detailed biochemical assays to evaluate its functional characteristics. Such studies are essential for developing predictive models of drug metabolism, improving drug efficacy, and minimizing toxicity, ultimately enhancing personalized medicine approaches. Additionally, Cyp3a16 may serve as a valuable model for studying the evolutionary adaptations of cytochrome P450 enzymes across different species. Overall, the research on Cyp3a16 recombinant protein is an important endeavor that bridges the gap between basic enzymology and practical applications in pharmacology and toxicology.
