Analytical Data
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基因名
NAGK
- Application
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别名
NAGK;N-acetyl-D-glucosamine kinase
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9UJ70
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表达区间
1-344aa
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氨基酸序列
MGSSHHHHHH SSGLVPRGSH MGSMAAIYGG VEGGGTRSEV LLVSEDGKIL AEADGLSTNH WLIGTDKCVE RINEMVNRAK RKAGVDPLVP LRSLGLSLSG GDQEDAGRIL IEELRDRFPY LSESYLITTD AAGSIATATP DGGVVLISGT GSNCRLINPD GSESGCGGWG HMMGDEGSAY WIAHQAVKIV FDSIDNLEAA PHDIGYVKQA MFHYFQVPDR LGILTHLYRD FDKCRFAGFC RKIAEGAQQG DPLSRYIFRK AGEMLGRHIV AVLPEIDPVL FQGKIGLPIL CVGSVWKSWE LLKEGFLLAL TQGREIQAQN FFSSFTLMKL RHSSALGGAS LGARHIGHLL PMDYSANAIA FYSYTFS
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分子量
40 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
N-acetylglucosamine kinase (NAGK) plays a critical role in the hexosamine biosynthetic pathway, which is essential for the post-translational modifications of proteins and the synthesis of glycoproteins and glycolipids. The enzyme catalyzes the phosphorylation of N-acetylglucosamine (GlcNAc) to N-acetylglucosamine-6-phosphate, a key intermediate for numerous cellular processes, including the regulation of gene expression and the maintenance of cellular homeostasis. Dysregulation of this pathway has been associated with metabolic disorders, such as diabetes and obesity, and has implications in cancer progression and neurodegenerative diseases. Consequently, understanding the structure and function of NAGK has garnered significant interest in various fields of biomedical research. Recent studies employ recombinant protein techniques to produce and purify NAGK, allowing for detailed kinetic and structural analyses. These investigations aim to elucidate the enzyme's catalytic mechanism and regulatory properties, providing insights that could lead to the development of specific inhibitors or modulators. As researchers continue to explore the potential therapeutic applications of targeting NAGK, the recombinant expression of this enzyme serves as a vital tool for drug discovery and the development of novel treatments for related diseases.
