Analytical Data
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基因名
MTHFSD
- Application
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别名
MTHFSD;Methenyltetrahydrofolate synthase domain-containing Protein
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q2M296-3
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表达区间
1-383aa
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氨基酸序列
MGSSHHHHHH SSGLVPRGSH MGSMEPRAVG VSKQDIREQI WGYMESQNLA DFPRPVHHRI PNFKGASHAA EQLPRLQAFK TARTIKVNPD APQKSARFFV LESKKTLLVP TPRLRTGLFN KITPPPGATK DILRKCATSQ GVRNYSVPIG LDSRVLVDLV VVGSVAVSEK GWRIGKGEGY ADLEYAMMVS MGAVSKETPV VTIVHDCQVV DIPEELVEEH DITVDYILTP TRVIATGCKR PKPMGITWFK ISLEMMEKIP ILRSLRAREQ QAGKDVTLQG EHQHLPEPGC QQTVPLSVGR RPPDTPGPET NSMEAAPGSP PGEGAPLAAD VYVGNLPGDA RVSDLKRALR ELGSVPLRLT WQGPRRRAFL HYPDSAAAQQ AVSCLQGLRL GTDTLRVALA RQQRDK
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分子量
45 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The study of MTHFSD (methylenetetrahydrofolate dehydrogenase 1) recombinant protein has gained prominence due to its critical role in folate metabolism and one-carbon metabolism pathways. MTHFSD catalyzes the conversion of methylenetetrahydrofolate to methenyl tetrahydrofolate, which is essential for synthesizing nucleotides and amino acids, thus influencing cell proliferation and growth. Its dysfunction has been implicated in various metabolic disorders and diseases, including certain cancers and neurological disorders. The recombinant expression of MTHFSD enables detailed investigations into its enzymatic properties, structural characteristics, and interactions with other biomolecules. Furthermore, understanding the structure-function relationship of this enzyme can lead to the development of pharmacological interventions aimed at restoring normal folate metabolism in patients. Advances in recombinant DNA technology allow for the large-scale production of MTHFSD, facilitating biochemical assays and structural studies that can illuminate the enzyme's mechanism of action and regulatory mechanisms. This research holds potential not only for basic science but also for therapeutic applications in treating folate-related diseases. Therefore, exploring recombinant MTHFSD provides significant insights into metabolic regulation and its implications for human health.
