Analytical Data
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Gene name
LCHN
- Application
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Alternative Names
DENND11; KIAA1147; LCHN; DENN domain-containing protein 11; DENND11; Protein LCHN
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
A4D1U4
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Expression Region
1-455aa
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AA Sequence
MVEQGDAAPL LRWAEGPAVS LPQAPQPQAG GWGRGGGGGA RPAAEPPRRR EPEEPAAPEV LLQPGRLELG DVEEDQVVAV FVVTFDPRSG NMVEWCLPQD IDLEGVEFKS MASGSHKIQS DFIYFRKGPF FGLACFANMP VESELERGAR MKSVGILSPS YTLLYRYMHF LENQVRHQLE MPGHYSHLAA FYEDKKGVLH AGPGRGSSLP PVYWLPSIHR YMYPEMKITH PAGCMSQFIK FFGEQILILW KFALLRKRIL IFSPPPVGVV CYRVYCCCCL ANVSLPGIGG TIPESKPFFY VNVADIESLE VEVSYVACTT EKIFEEKREL YDVYVDNQNV KTHHDHLQPL LKINSADREK YRRLNEQRQM LLYSQEVEED YNPCEEDLFV LFFLEQNNRI FQTLLEVSAS QDKTLTAEHA RGMGLDPQGD RSFLLDLLEA YGIDVMLVID NPCCP
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Molecular Weight
51.4 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
LCHN, also known as lactate dehydrogenase C (LDH-C), is a pivotal enzyme involved in the conversion of lactate to pyruvate during anaerobic metabolism, primarily in the testis and spermatozoa. Research into LCHN recombinant proteins has garnered significant interest due to their potential roles in reproductive biology and energy metabolism in male fertility. The unique expression of LCHN in germ cells highlights its importance in spermatogenesis and sperm maturation, making it a valuable target for studying male infertility and contraceptive methods. Moreover, understanding the structural and functional properties of LCHN through recombinant protein technology can provide insights into its enzymatic mechanisms and regulatory pathways. Advances in protein engineering and expression systems have facilitated the production of LCHN, enabling detailed investigations into its biochemical characteristics and interactions. This research has implications not only for reproductive health but also for developing therapeutics targeting metabolic diseases where lactate metabolism is disrupted. Overall, the investigation of LCHN recombinant proteins is a promising avenue for enhancing our understanding of energy metabolism in reproductive functions and addressing male reproductive health challenges.
