Analytical Data
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基因名
LCHN
- Application
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别名
DENND11; KIAA1147; LCHN; DENN domain-containing protein 11; DENND11; Protein LCHN
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
A4D1U4
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表达区间
1-455aa
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氨基酸序列
MVEQGDAAPL LRWAEGPAVS LPQAPQPQAG GWGRGGGGGA RPAAEPPRRR EPEEPAAPEV LLQPGRLELG DVEEDQVVAV FVVTFDPRSG NMVEWCLPQD IDLEGVEFKS MASGSHKIQS DFIYFRKGPF FGLACFANMP VESELERGAR MKSVGILSPS YTLLYRYMHF LENQVRHQLE MPGHYSHLAA FYEDKKGVLH AGPGRGSSLP PVYWLPSIHR YMYPEMKITH PAGCMSQFIK FFGEQILILW KFALLRKRIL IFSPPPVGVV CYRVYCCCCL ANVSLPGIGG TIPESKPFFY VNVADIESLE VEVSYVACTT EKIFEEKREL YDVYVDNQNV KTHHDHLQPL LKINSADREK YRRLNEQRQM LLYSQEVEED YNPCEEDLFV LFFLEQNNRI FQTLLEVSAS QDKTLTAEHA RGMGLDPQGD RSFLLDLLEA YGIDVMLVID NPCCP
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分子量
51.4 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
LCHN, also known as lactate dehydrogenase C (LDH-C), is a pivotal enzyme involved in the conversion of lactate to pyruvate during anaerobic metabolism, primarily in the testis and spermatozoa. Research into LCHN recombinant proteins has garnered significant interest due to their potential roles in reproductive biology and energy metabolism in male fertility. The unique expression of LCHN in germ cells highlights its importance in spermatogenesis and sperm maturation, making it a valuable target for studying male infertility and contraceptive methods. Moreover, understanding the structural and functional properties of LCHN through recombinant protein technology can provide insights into its enzymatic mechanisms and regulatory pathways. Advances in protein engineering and expression systems have facilitated the production of LCHN, enabling detailed investigations into its biochemical characteristics and interactions. This research has implications not only for reproductive health but also for developing therapeutics targeting metabolic diseases where lactate metabolism is disrupted. Overall, the investigation of LCHN recombinant proteins is a promising avenue for enhancing our understanding of energy metabolism in reproductive functions and addressing male reproductive health challenges.
