Analytical Data
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基因名
SPTLC2
- Application
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别名
SPTLC2；KIAA0526；LCB2；Serine palmitoyltransferase 2
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O15270
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表达区间
88-562aa
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氨基酸序列
RDFLRYWRIEKCHHATEREEQKDFVSLYQDFENFYTRNLYMRIRDNWNRPICSVPGARVDIMERQSHDYNWSFKYTGNIIKGVINMGSYNYLGFARNTGSCQEAAAKVLEEYGAGVCSTRQEIGNLDKHEELEELVARFLGVEAAMAYGMGFATNSMNIPALVGKGCLILSDELNHASLVLGARLSGATIRIFKHNNMQSLEKLLKDAIVYGQPRTRRPWKKILILVEGIYSMEGSIVRLPEVIALKKKYKAYLYLDEAHSIGALGPTGRGVVEYFGLDPEDVDVMMGTFTKSFGASGGYIGGKKELIDYLRTHSHSAVYATSLSPPVVEQIITSMKCIMGQDGTSLGKECVQQLAENTRYFRRRLKEMGFIIYGNEDSPVVPLMLYMPAKIGAFGREMLKRNIGVVVVGFPATPIIESRARFCLSAAHTKEILDTALKEIDEVGDLLQLKYSRHRLVPLLDRPFDETTYEETED
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分子量
69.7kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The research on SPTLC2 recombinant protein focuses on its crucial role in sphingolipid metabolism, a fundamental process in cellular signaling and membrane integrity. SPTLC2, or serine palmitoyltransferase, large subunit 2, is one of the key components in the synthesis of sphingolipids, which are vital for various cellular functions, including apoptosis, differentiation, and inflammation. Mutations in the SPTLC2 gene are linked to several neurological disorders, such as hereditary sensory and autonomic neuropathy (HSAN) and other sphingolipid-related diseases. The recombinant expression of SPTLC2 not only facilitates the understanding of its biochemical properties and regulatory mechanisms but also provides a platform for studying its pathophysiological implications. By generating this protein in a controlled laboratory environment, researchers can explore its interactions with other cellular components and assess how alterations in its activity contribute to disease. Additionally, studying the structure-function relationships of SPTLC2 may pave the way for the development of therapeutic strategies aimed at mitigating the effects of mutations associated with sphingolipid metabolism disorders. These investigations are crucial for expanding our understanding of sphingolipid biology and developing potential interventions to treat diseases linked to this pathway.
