Analytical Data
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基因名
MMACHC
- Application
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别名
MMACHC;Cyanocobalamin reductase / alkylcobalamin dealkylase
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9Y4U1
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表达区间
1-282aa
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氨基酸序列
MGSSHHHHHH SSGLVPRGSH MGSHMEPKVA ELKQKIEDTL CPFGFEVYPF QVAWYNELLP PAFHLPLPGP TLAFLVLSTP AMFDRALKPF LQSCHLRMLT DPVDQCVAYH LGRVRESLPE LQIEIIADYE VHPNRRPKIL AQTAAHVAGA AYYYQRQDVE ADPWGNQRIS GVCIHPRFGG WFAIRGVVLL PGIEVPDLPP RKPHDCVPTR ADRIALLEGF NFHWRDWTYR DAVTPQERYS EEQKAYFSTP PAQRLALLGL AQPSEKPSSP SPDLPFTTPA PKKPGNPSRA RSWLSPRVSP PASPGP
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分子量
34 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
MMACHC (Methylmalonic Aciduria and Homocystinuria, CblC Type) is a crucial enzyme involved in the metabolism of vitamin B12 (cobalamin), playing an essential role in cellular processes. Deficiencies in MMACHC activity lead to a metabolic disorder characterized by elevated levels of methylmalonic acid and homocysteine in the body, resulting in severe neurological and developmental issues. Understanding the structure and function of the MMACHC protein is vital for developing potential treatments for patients affected by this condition. Recent research has focused on the recombinant expression of MMACHC, which allows for the study of its biochemical properties, interactions with other cellular components, and the mechanisms of cobalamin-related metabolic pathways. Furthermore, this approach provides insights into the molecular basis of MMACHC deficiency, aiding in the design of targeted therapies and improving diagnostic techniques. The development of recombinant MMACHC also paves the way for potential gene therapy strategies and enzyme replacement therapies, which could significantly alleviate the clinical symptoms associated with MMACHC-related disorders. By elucidating the structure-function relationships of this protein, researchers aspire to contribute to novel therapeutic innovations that could enhance the quality of life for affected individuals.
