Analytical Data
-
基因名
DGKa
- Application
-
别名
DGKa；DAGK；DAGK1；Diacylglycerol kinase alpha
-
种属
Human
-
表达系统
E. coli
-
标签
His tag N-Terminus
-
纯度
Greater than 90% as determined by SDS-PAGE.
-
蛋白编号
P23743
-
表达区间
1-735aa
-
氨基酸序列
MAKERGLISPSDFAQLQKYMEYSTKKVSDVLKLFEDGEMAKYVQGDAIGY EGFQQFLKIYLEVDNVPRHLSLALFQSFETGHCLNETNVTKDVVCLNDVS CYFSLLEGGRPEDKLEFTFKLYDTDRNGILDSSEVDKIILQMMRVAEYLD WDVSELRPILQEMMKEIDYDGSGSVSQAEWVRAGATTVPLLVLLGLEMTL KDDGQHMWRPKRFPRPVYCNLCESSIGLGKQGLSCNLCKYTVHDQCAMKA LPCEVSTYAKSRKDIGVQSHVWVRGGCESGRCDRCQKKIRIYHSLTGLHC VWCHLEIHDDCLQAVGHECDCGLLRDHILPPSSIYPSVLASGPDRKNSKT SQKTMDDLNLSTSEALRIDPVPNTHPLLVFVNPKSGGKQGQRVLWKFQYI LNPRQVFNLLKDGPEIGLRLFKDVPDSRILVCGGDGTVGWILETIDKANL PVLPPVAVLPLGTGNDLARCLRWGGGYEGQNLAKILKDLEMSKVVHMDRW SVEVIPQQTEEKSDPVPFQIINNYFSIGVDASIAHRFHIMREKYPEKFNS RMKNKLWYFEFATSESIFSTCKKLEESLTVEICGKPLDLSNLSLEGIAVL NIPSMHGGSNLWGDTRRPHGDIYGINQALGATAKVITDPDILKTCVPDLS DKRLEVVGLEGAIEMGQIYTKLKNAGRRLAKCSEITFHTTKTLPMQIDGE PWMQTPCTIKITHKNQMPMLMGPPPRSTNFFGFLS
-
分子量
105 kDa
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Diacylglycerol kinase alpha (DGKa) is an important enzyme involved in the regulation of cellular signaling pathways through its role in the phosphorylation of diacylglycerol (DAG) to produce phosphatidic acid (PA). This enzymatic reaction plays a crucial role in modulating various physiological processes, including cell proliferation, differentiation, and survival, as well as in the immune response. Dysregulation of DGKa has been implicated in several diseases, including cancer, autoimmune disorders, and neurodegenerative diseases, highlighting its potential as a therapeutic target. Research into DGKa involves the production of recombinant proteins, which allows for detailed biochemical characterization, structural studies, and the examination of its regulatory mechanisms. The ability to generate recombinant DGKa enables scientists to study its enzymatic activity, interaction with other signaling molecules, and the effects of post-translational modifications. Moreover, understanding the structure-function relationship of DGKa can provide insights into its role in pathological conditions and aid in the development of novel drugs that could modulate its activity for therapeutic benefits. Overall, the study of DGKa and its recombinant forms is vital for unraveling the complexities of cellular signaling and for identifying new avenues for intervention in disease.
