Analytical Data
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Gene name
MAPK3
- Application
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Alternative Names
MAPKAPK3;MAP kinase-activated Protein kinase 3
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P27361
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Expression Region
2-379aa
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AA Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSAAAAAQGGGGGEPRRTEGV GPGVPGEVEMVKGQPFDVGPRYTQLQYIGEGAYGMVSSAYDHVRKTRVAI KKISPFEHQTYCQRTLREIQILLRFRHENVIGIRDILRASTLEAMRDVYI VQDLMETDLYKLLKSQQLSNDHICYFLYQILRGLKYIHSANVLHRDLKPS NLLSNTTCDLKICDFGLARIADPEHDHTGFLTEYVATRWYRAPEIMLNSK GYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGILGSPSQEDLN CIINMKARNYLQSLPSKTKVAWAKLFPKSDSKALDLLDRMLTFNPNKRIT VEEALAHPYLEQYYDPTDEPVAEEPFTFAMELDDLPKERLKELIFQETAR FQPGVLEAP
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Molecular Weight
70 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Mitogen-activated protein kinase 3 (MAPK3), also known as extracellular signal-regulated kinase 1 (ERK1), is a critical component of the MAPK signaling pathway, which regulates various cellular processes including proliferation, differentiation, and survival in response to extracellular stimuli. The MAPK pathway is essential for translating environmental signals into cellular responses, and its dysregulation is implicated in numerous diseases, particularly cancer. Research into MAPK3 has gained prominence due to its role in mediating the effects of growth factors and other mitogens on cell function. Recombinant MAPK3 protein has been utilized extensively in both in vitro and in vivo studies to elucidate its biological functions and intricate regulatory mechanisms. Furthermore, recombinant MAPK3 has been instrumental in developing novel therapeutic strategies targeting MAPK pathways, with a focus on inhibiting aberrant signaling in cancer cells. The expression and purification of active recombinant MAPK3 allow researchers to perform kinetic analyses and screen for potential inhibitors, paving the way for new treatments. Additionally, understanding the post-translational modifications and interactions of MAPK3 can reveal critical insights into its role in cellular signaling networks. Given its central role in key physiological processes and its involvement in various pathologies, MAPK3 remains a compelling target for biomedical research and drug development.
