Analytical Data
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基因名
map
- Application
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别名
MAP4;Microtubule-associated Protein 4
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种属
E.coli
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P0AE18
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表达区间
1-264aa
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氨基酸序列
MGSSHHHHHHSSGLVPRGSHMAISIKTPEDIEKMRVAGRLAAEVLEMIEP YVKPGVSTGELDRICNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHG IPDDAKLLKDGDIVNIDVTVIKDGFHGDTSKMFIVGKPTIMGERLCRITQ ESLYLALRMVKPGINLREIGAAIQKFVEAEGFSVVREYCGHGIGRGFHEE PQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMKDGWTVKTKDRSL SAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE
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分子量
32 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The research on MAP (microtubule-associated protein) recombinant proteins has gained significant attention due to their crucial role in cellular processes, particularly in neurons, where they are involved in the stabilization of microtubules and the regulation of neurofilament organization. MAPs, such as tau, are key regulators of microtubule dynamics, affecting cellular structure and intracellular transport. Dysregulation of MAPs is linked to various neurodegenerative diseases, including Alzheimer's disease, where hyperphosphorylated tau protein forms neurofibrillary tangles. To better understand their function and implications in disease, scientists have developed recombinant MAP proteins, allowing for detailed studies of their biophysical properties, interactions, and effects on microtubule assembly. Utilizing recombinant technology enables researchers to manipulate these proteins for experimental purposes, providing insights into their role in cellular contexts and the potential for therapeutic interventions. The ability to produce MAPs in vitro also facilitates high-throughput screening for molecules that can modulate their activity, advancing our understanding of their biological significance and paving the way for drug discovery targeting MAP-related pathologies.
