Analytical Data
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Gene name
KLHL32
- Application
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Alternative Names
KLHL32; BKLHD5; KIAA1900Kelch-like protein 32; BTB and kelch domain-containing protein 5
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q96NJ5
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Expression Region
1-470aa
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AA Sequence
MPSERCLSIQEMLTGQRLCHSESHNDSVLAALNQQRSDGILCDITLIAEEQKFHAHKAVLAACSDYFRAMFSLCMVESGADEVNLHGVTSLGLKQALEFAYTGQILLEPGVIQDVLAAGSHLQLLELLNLCSHYLIQELNSFNYLDLYRLADLFNLTLLEKAVIDFLVKHLSELLKSRPEEVLTLPYCLLQEVLKSDRLTSLSEEQIWQLAVRWLEHNCHYQYMDELLQYIRFGLMDVDTLHTVALSHPLVQASETATALVNEALEYHQSIYAQPVWQTRRTKPRFQSDTLYIIGGKKREVCKVKELRYFNPVDQENALIAAIANWSELAPMPVGRSHHCVAVMGDFLFVAGGEVEHASGRTCAVRTACRYDPRSNSWAEIAPMKNCREHFVLGAMEEYLYAVGGRNELRQVLPTVERYCPKKNKWTFVQSFDRSLSCHAGYVADGLLWISGRTYLMLDLSKHTFIVVYI
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Molecular Weight
79.8 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
KLHL32, a member of the BTB-Kelch protein family, has gained attention in the field of molecular biology due to its potential roles in cellular processes such as protein degradation, regulation of signaling pathways, and involvement in various diseases. Its unique structure, characterized by a BTB domain for dimerization and Kelch repeats that mediate protein-protein interactions, positions KLHL32 as a key player in ubiquitin-proteasome pathways. Research has suggested that KLHL32 may be implicated in neuronal development and function, with aberrations in its expression linked to neurodegenerative disorders and cancer. The mounting evidence of KLHL32's involvement in these critical cellular mechanisms has prompted investigations into its biochemical properties and interactions. Understanding the functional dynamics of KLHL32 not only offers insights into fundamental biological processes but also holds promise for the development of novel therapeutic strategies targeting its pathways in various pathologies. Overall, the study of KLHL32 as a recombinant protein provides an important avenue for elucidating its role in health and disease, potentially leading to advancements in targeted treatments and biomedical applications.
