Analytical Data
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基因名
KLHL20
- Application
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别名
KLHL20. KLEIP. KLHLX
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9Y2M5
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表达区间
1-609aa
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氨基酸序列
MEGKPMRRCTNIRPGETGMDVTSRCTLGDPNKLPEGVPQPARMPYISDKHPRQTLEVINLLRKHRELCDVVLVVGAKKIYAHRVILSACSPYFRAMFTGELAESRQTEVVIRDIDERAMELLIDFAYTSQITVEEGNVQTLLPAACLLQLAEIQEACCEFLKRQLDPSNCLGIRAFADTHSCRELLRIADKFTQHNFQEVMESEEFMLLPANQLIDIISSDELNVRSEEQVFNAVMAWVKYSIQERRPQLPQVLQHVRLPLLSPKFLVGTVGSDPLIKSDEECRDLVDEAKNYLLLPQERPLMQGPRTRPRKPIRCGEVLFAVGGWCSGDAISSVERYDPQTNEWRMVASMSKRRCGVGVSVLDDLLYAVGGHDGSSYLNSVERYDPKTNQWSSDVAPTSTCRTSVGVAVLGGFLYAVGGQDGVSCLNIVERYDPKENKWTRVASMSTRRLGVAVAVLGGFLYAVGGSDGTSPLNTVERYNPQENRWHTIAPMGTRRKHLGCAVYQDMIYAVGGRDDTTELSSAERYNPRTNQWSPVVAMTSRRSGVGLAVVNGQLMAVGGFDGTTYLKTIEVFDPDANTWRLYGGMNYRRLGGGVGVIKMTHCESHIW
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分子量
94.4 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
KLHL20, a member of the Kelch-like protein family, has garnered attention in recent years due to its potential involvement in various cellular processes, including protein degradation, cellular signaling, and cytoskeletal organization. Research has shown that KLHL20 plays a critical role in the ubiquitin-proteasome system, where it functions as a substrate recognition component for the E3 ubiquitin ligase complex, facilitating the degradation of specific target proteins. Its association with several diseases, particularly neurodegenerative disorders, has prompted scientists to investigate its role in maintaining cellular homeostasis and regulating protein levels. Furthermore, KLHL20 has been implicated in developmental processes and may be essential for proper neuronal differentiation and function. As a result, the recombinant expression and characterization of KLHL20 have become pivotal for understanding its biological functions and mechanisms of action. By producing and analyzing KLHL20 in vitro, researchers aim to elucidate its structural properties, binding interactions, and functional roles, ultimately contributing to our comprehension of its involvement in disease pathogenesis and identifying potential therapeutic targets. The study of KLHL20's recombinant protein thus serves as a foundational step in deciphering the complexities of protein regulation and cellular dynamics, highlighting its significance in both basic research and clinical applications.
