Analytical Data
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Gene name
VD3
- Application
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Alternative Names
VD3；CYP1ALPHA；CYP27B；25-hydroxyvitamin D-1 alpha hydroxylase. mitochondrial
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O15528
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Expression Region
1-508aa
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AA Sequence
MTQTLKYASRVFHRVRWAPELGASLGYREYHSARRSLADIPGPSTPSFLAELFCKGGLSRLHELQVQGAAHFGPVWLASFGTVRTVYVAAPALVEELLRQEGPRPERCSFSPWTEHRRCRQRACGLLTAEGEEWQRLRSLLAPLLLRPQAAARYAGTLNNVVCDLVRRLRRQRGRGTGPPALVRDVAGEFYKFGLEGIAAVLLGSRLGCLEAQVPPDTETFIRAVGSVFVSTLLTMAMPHWLRHLVPGPWGRLCRDWDQMFAFAQRHVERREAEAAMRNGGQPEKDLESGAHLTHFLFREELPAQSILGNVTELLLAGVDTVSNTLSWALYELSRHPEVQTALHSEITAALSPGSSAYPSATVLSQLPLLKAVVKEVLRLYPVVPGNSRVPDKDIHVGDYIIPKNTLVTLCHYATSRDPAQFPEPNSFRPARWLGEGPTPHPFASLPFGFGKRSCMGRRLAELELQMALAQILTHFEVQPEPGAAPVRPKTRTVLVPERSINLQFLDR
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Molecular Weight
56.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
VD3 recombinant protein, also known as vitamin D3 recombinant protein, has gained significant attention in the field of biomedical research due to its crucial role in calcium metabolism and immune system regulation. Vitamin D3, primarily synthesized in the skin through sunlight exposure, is essential for maintaining bone health and preventing diseases such as osteoporosis and rickets. However, many individuals suffer from vitamin D deficiency due to limited sun exposure, dietary insufficiency, or certain health conditions. The development of VD3 recombinant protein offers a promising avenue for therapeutic interventions, providing a controlled and consistent source of vitamin D3 for supplementation. This approach enables researchers to explore its effects on various physiological processes, including cellular differentiation, immune response, and potential anti-inflammatory properties. In recent years, studies have also investigated the implications of VD3 recombinant protein in the context of chronic diseases, such as diabetes, cardiovascular disorders, and autoimmunity, underscoring its potential as a versatile therapeutic agent. Moreover, the advancement of recombinant DNA technology has facilitated the production of this protein, ensuring higher purity and bioavailability compared to traditional supplementation methods. Overall, the research surrounding VD3 recombinant protein emphasizes its importance not only in addressing nutrient deficiencies but also in its potential role in enhancing health outcomes across different populations.
