Analytical Data
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Gene name
KLHL1
- Application
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Alternative Names
FLJ30047; Kelch (Drosophila) like 1; Kelch like 1 (Drosophila); Kelch like 1; Kelch like protein 1; kelch-like family member 1; Kelch-like protein 1; KIAA1490; KLHL1; KLHL1_HUMAN; Mayven related protein 2; MRP2; OTTHUMP00000018492; OTTHUMP00000040834
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9NR64
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Expression Region
1-748aa
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AA Sequence
MSGSGRKDFD VKHILRLRWK LFSHPSPSTG GPAGGGCLQQ DGSGSFEHWG PSQSRLLKSQ ERSGVSTFWK KPSSSSSSSS SPSSSSSSFN PLNGTLLPVA TRLQQGAPGQ GTQQPARTLF YVESLEEEVV PGMDFPGPHE KGLVLQELKV EPDNSSQATG EGCGHRLSST GHSMTPQSDL DSSSSEEFYQ AVHHAEQTFR KMESYLKQQQ LCDVILIVGN RKIPAHRLVL SSVSDYFAAM FTSDVCEAKQ EEIKMEGIDP NALWDLVQFA YTGCLELKED TIENLLAAAC LLQLPQVVEV CCHFLMKLLH PSNCLGIRAF ADAQGCIELM KVAHSYTMEN IMEVIRNQEF LLLPAEELHK LLASDDVNVP DEETIFHALM MWVKYDMQSR CNDLSMLLAF IRLPLLPPQI LADLENHALF KNDLECQKLI LEAMKYHLLP ERRTLMQSPR TKPRKSTVGT LYAVGGMDNN KGATTIEKYD LRTNLWIQAG MMNGRRLQFG VAVIDDKLFV IGGRDGLKTL NTVECYNPKT KTWTVLPPMS THRHGLGVTV LEGPIYAVGG HDGWSYLNTV ERWDPQSQQW TFVASMSIAR STVGVAALNG KLYSVGGRDG SSCLSSMEYY DPHTNKWNMC APMCKRRGGV GVATCDGFLY AVGGHDAPAS NHCSRLLDYV ERYDPKTDTW TMVAPLSMPR DAVGVCLLGD RLYAVGGYDG QTYLNTMESY DPQTNEWTQM ASLNIGRAGA CVVVIKQP
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Molecular Weight
82.6 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
KLHL1, a member of the BTB-Kelch protein family, has emerged as a significant focus of research due to its role in various cellular processes including protein degradation, cytoskeletal organization, and signal transduction. The protein is characterized by its unique structure, consisting of a BTB domain that facilitates protein-protein interactions and a Kelch repeat domain that typically mediates substrate recognition for ubiquitination. Mutations in the KLHL1 gene have been linked to several human diseases, particularly neurological disorders and congenital myopathies, highlighting its importance in maintaining cellular homeostasis. The recombinant expression of KLHL1 has been pursued to better understand its functional mechanisms and interactions within cellular pathways. Researchers aim to elucidate its role in ubiquitin-proteasome degradation pathways, which could have implications for the development of therapeutic strategies targeting diseases associated with KLHL1 dysregulation. Furthermore, KLHL1 has been shown to interact with various substrates, further complicating the understanding of its biological functions. As such, the production of recombinant KLHL1 protein allows for the exploration of its biochemical properties and interaction dynamics, providing insights that could lead to potential advancements in medical research and therapeutic interventions. Overall, KLHL1 represents an intriguing target for further investigation given its pivotal role in cellular processes and its association with human health and disease.
