Analytical Data
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Gene name
KRT5
- Application
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Alternative Names
KRT85;KRTHB5;Keratin. type II cuticular Hb5
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P13647
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Expression Region
1-590aa
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AA Sequence
MSRQSSVSFRSGGSRSFSTASAITPSVSRTSFTSVSRSGGGGGGGFGRVS LAGACGVGGYGSRSLYNLGGSKRISISTSGGSFRNRFGAGAGGGYGFGGG AGSGFGFGGGAGGGFGLGGGAGFGGGFGGPGFPVCPPGGIQEVTVNQSLL TPLNLQIDPSIQRVRTEEREQIKTLNNKFASFIDKVRFLEQQNKVLDTKW TLLQEQGTKTVRQNLEPLFEQYINNLRRQLDSIVGERGRLDSELRNMQDL VEDFKNKYEDEINKRTTAENEFVMLKKDVDAAYMNKVELEAKVDALMDEI NFMKMFFDAELSQMQTHVSDTSVVLSMDNNRNLDLDSIIAEVKAQYEEIA NRSRTEAESWYQTKYEELQQTAGRHGDDLRNTKHEISEMNRMIQRLRAEI DNVKKQCANLQNAIADAEQRGELALKDARNKLAELEEALQKAKQDMARLL REYQELMNTKLALDVEIATYRKLLEGEECRLSGEGVGPVNISVVTGSVSS GYGSGSGYGGGLGGGLGGGLGGGLAGGGSGSYYSSSSGGVGLSGGLSVGG SGFSASSGRGLGVGFGSGGGSSSSVKFVSTTSSSRKSFKS
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Molecular Weight
91 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
KRT5, or Keratin 5, is a type of intermediate filament protein primarily expressed in the basal layer of epithelial tissues, particularly in stratified epithelia such as the skin, hair follicles, and esophagus. Its primary role is to provide structural integrity and mechanical resilience to epithelial cells, thus playing a crucial part in tissue development and homeostasis. Research into KRT5 recombinant proteins has gained importance due to their potential applications in regenerative medicine, wound healing, and the treatment of skin disorders. The recombinant expression of KRT5 allows for the production of large quantities of the protein, enabling detailed studies of its biochemical properties, cellular functions, and interactions with other keratins and cytoskeletal elements. Furthermore, understanding the role of KRT5 in epithelial differentiation and its involvement in skin pathologies, such as epidermolysis bullosa and other keratinization disorders, has opened avenues for potential therapeutic interventions. Biochemical analysis and structural studies of KRT5 recombinant proteins are pivotal for deciphering the molecular mechanisms underlying epithelial integrity and disease, contributing to the development of targeted therapies and advanced biomaterials in dermatology and tissue engineering.
