Cat: PA1000-1761

Recombinant Human KRT20 Protein,His

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Analytical Data

  • Gene name

    KRT20

  • Application

    SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

  • Alternative Names

    KRT20;Keratin. type I cytoskeletal 20

  • Species

    Human

  • Source

    E. coli

  • Tag

    His tag N-Terminus

  • Purity

    Greater than 90% as determined by SDS-PAGE.

  • Uniprot

    P35900

  • Expression Region

    2-424aa

  • AA Sequence

    MASMTGGQQMGRGHHHHHHGNLYFQGEFDFSRRSFHRSLSSSLQAPVVST VGMQRLGTTPSVYGGAGGRGIRISNSRHTVNYGSDLTGGGDLFVGNEKMA MQNLNDRLASYLEKVRTLEQSNSKLEVQIKQWYETNAPRAGRDYSAYYRQ IEELRSQIKDAQLQNARCVLQIDNAKLAAEDFRLKYETERGIRLTVEADL QGLNKVFDDLTLHKTDLEIQIEELNKDLALLKKEHQEEVDGLHKHLGNTV NVEVDAAPGLNLGVIMNEMRQKYEVMAQKNLQEAKEQFERQTAVLQQQVT VNTEELKGTEVQLTELRRTSQSLEIELQSHLSMKESLEHTLEETKARYSS QLANLQSLLSSLEAQLMQIRSNMERQNNEYHILLDIKTRLEQEIATYRRL LEGEDVKTTEYQLSTLEERDIKKTRKIKTVVQEVVDGKVVSSEVKEVEEN I

  • Molecular Weight

    52 kDa

  • Endotoxin

    < 1.0 EU per μg protein as determined by the LAL method.

  • Form

    Freeze-dried powder

  • Buffer formulation

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • Reconstitution

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • Customization

    Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

  • Stability Test

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • Storage & Shelf Life

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • Shipping

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

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Protein Description

KRT20, or Keratin 20, is a member of the keratin family, primarily expressed in the gastrointestinal epithelium and serves as a critical marker for intestinal cell differentiation and function. The study of KRT20 has gained significant importance due to its potential roles in various diseases, particularly in cancers of the digestive system. Altered expression levels of KRT20 have been associated with the progression and prognosis of colorectal cancer, where it may serve as a biomarker for diagnosis and therapeutic strategies. Additionally, research into KRT20 recombinant proteins aims to elucidate its biological functions, interactions with other cellular components, and its mechanistic roles in cancer biology. The production of KRT20 as a recombinant protein facilitates in vitro studies, providing insights into its structural properties and functional implications. Understanding these dynamics could pave the way for novel diagnostic tools or targeted treatments, highlighting the necessity for continued research in this area.

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