Analytical Data
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基因名
KRT20
- Application
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别名
KRT20;Keratin. type I cytoskeletal 20
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P35900
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表达区间
2-424aa
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氨基酸序列
MASMTGGQQMGRGHHHHHHGNLYFQGEFDFSRRSFHRSLSSSLQAPVVST VGMQRLGTTPSVYGGAGGRGIRISNSRHTVNYGSDLTGGGDLFVGNEKMA MQNLNDRLASYLEKVRTLEQSNSKLEVQIKQWYETNAPRAGRDYSAYYRQ IEELRSQIKDAQLQNARCVLQIDNAKLAAEDFRLKYETERGIRLTVEADL QGLNKVFDDLTLHKTDLEIQIEELNKDLALLKKEHQEEVDGLHKHLGNTV NVEVDAAPGLNLGVIMNEMRQKYEVMAQKNLQEAKEQFERQTAVLQQQVT VNTEELKGTEVQLTELRRTSQSLEIELQSHLSMKESLEHTLEETKARYSS QLANLQSLLSSLEAQLMQIRSNMERQNNEYHILLDIKTRLEQEIATYRRL LEGEDVKTTEYQLSTLEERDIKKTRKIKTVVQEVVDGKVVSSEVKEVEEN I
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分子量
52 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
KRT20, or Keratin 20, is a member of the keratin family, primarily expressed in the gastrointestinal epithelium and serves as a critical marker for intestinal cell differentiation and function. The study of KRT20 has gained significant importance due to its potential roles in various diseases, particularly in cancers of the digestive system. Altered expression levels of KRT20 have been associated with the progression and prognosis of colorectal cancer, where it may serve as a biomarker for diagnosis and therapeutic strategies. Additionally, research into KRT20 recombinant proteins aims to elucidate its biological functions, interactions with other cellular components, and its mechanistic roles in cancer biology. The production of KRT20 as a recombinant protein facilitates in vitro studies, providing insights into its structural properties and functional implications. Understanding these dynamics could pave the way for novel diagnostic tools or targeted treatments, highlighting the necessity for continued research in this area.
