Analytical Data
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Gene name
algL
- Application
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Alternative Names
algL；Alginate lyase
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O52195
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Expression Region
24-374aa
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AA Sequence
AEALVPPKGYYAPVDIRKGEAPACPVVPEPFTGELVFRSKYEGSDAARST LNEEAEKAFRTKTAPITQIERGVSRMVMRYMEKGRAGDLECTLAWLDAWA EDGALLTTEYNHTGKSMRKWALGSLAGAYLRLKFSSSQPLAAYPEQARRI ESWFAKVGDQVIKDWSDLPLKRINNHSYWAAWAVMAAGVATNRRPLFDWA VEQFHIAAGQVDSNGFLPNELKRRQRALAYHNYSLPPLMMVAAFALANGV DLRGDNDGALGRLAGNVLAGVEKPEPFAERAGDEDQDMEDLETDAKFSWL EPYCALYSCSPALRERKAEMGPFKNFRLGGDVTRIFDPAEKSPRSTVGKR D
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Molecular Weight
59 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
AlgL, a key enzyme in the bacterial alginate degradation pathway, plays a significant role in the metabolism of alginate, a polysaccharide found in the cell walls of brown algae and produced by certain bacteria. The study of AlgL has gained importance due to its potential applications in biotechnology and environmental sustainability. Alginate is widely used in food, pharmaceuticals, and biomedicine, and its degradation can be harnessed for bioconversion processes. Understanding the structure and function of AlgL can lead to advancements in bioremediation technologies, enabling more efficient breakdown of alginate-rich waste. Moreover, the recombinant production of AlgL allows for detailed kinetic studies and structure-function analyses, enhancing our knowledge of polypeptide interactions and enzyme mechanisms. Research has also focused on the gene regulation of alginate degradation, which can provide insights into the adaptive strategies of bacteria in different nutrient environments. Thus, AlgL serves as a model enzyme for studying polysaccharide lyases and their potential exploitation in various industrial applications, making it a subject of growing interest in enzymology and biotechnology research.
