Analytical Data
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基因名
KLF4
- Application
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别名
KLF4;EZF;GKLF;Krueppel-like factor 4
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O43474-1
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表达区间
1-479aa
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氨基酸序列
MRQPPGESDMAVSDALLPSFSTFASGPAGREKTLRQAGAPNNRWREELSH MKRLPPVLPGRPYDLAAATVATDLESGGAGAACGGSNLAPLPRRETEEFN DLLDLDFILSNSLTHPPESVAATVSSSASASSSSSPSSSGPASAPSTCSF TYPIRAGNDPGVAPGGTGGGLLYGRESAPPPTAPFNLADINDVSPSGGFV AELLRPELDPVYIPPQQPQPPGGGLMGKFVLKASLSAPGSEYGSPSVISV SKGSPDGSHPVVVAPYNGGPPRTCPKIKQEAVSSCTHLGAGPPLSNGHRP AAHDFPLGRQLPSRTTPTLGLEEVLSSRDCHPALPLPPGFHPHPGPNYPS FLPDQMQPQVPPLHYQELMPPGSCMPEEPKPKRGRRSWPRKRTATHTCDY AGCGKTYTKSSHLKAHLRTHTGEKPYHCDWDGCGWKFARSDELTRHYRKH TGHRPFQCQKCDRAFSRSDHLALHMKRHFESGGGGSPGRRRRRRRRRRR
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分子量
53 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
KLF4, or Kruppel-like factor 4, is a crucial transcription factor involved in various biological processes, including cell differentiation, proliferation, and maintaining pluripotency in stem cells. Its role in regulating gene expression makes it a significant focus of biological research, particularly in developmental biology and regenerative medicine. KLF4 is known to play a key role in the reprogramming of somatic cells into induced pluripotent stem cells (iPSCs), making it a vital component in research related to cellular reprogramming and therapeutic applications. Additionally, KLF4 has been implicated in various diseases, including cancer, where it may function as a tumor suppressor or oncogene depending on the context. This dual role highlights the complexity of KLF4's function in cell biology. The development of recombinant KLF4 protein has facilitated in-depth studies of its structure, function, and interactions with other molecular partners. Researchers utilize KLF4 recombinant proteins to investigate its mechanism of action, potential binding partners, and downstream effects in cellular processes. Furthermore, understanding KLF4's role in pathological conditions could pave the way for novel therapeutic interventions, particularly in cancer therapy and regenerative medicine. Consequently, the study of KLF4, especially through the lens of recombinant protein technology, is a burgeoning field that promises to enhance our understanding of cellular mechanisms and contribute to the advancement of medical science.
