Analytical Data
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基因名
Shh
- Application
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q15465
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表达区间
24-197aa
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氨基酸序列
CGPGRGFGKRRHPKKLTPLAYKQFIPNVAEKTLGASGRYEGKISRNSERF KELTPNYNPDIIFKDEENTGADRLMTQRCKDKLNALAISVMNQWPGVKLR VTEGWDEDGHHSEESLHYEGRAVDITTSDRDRSKYGMLARLAVEAGFDWV YYESKAHIHCSVKAENSVAAKSGG
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分子量
51 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The study of Sonic Hedgehog (Shh) recombinant protein has gained significant attention in developmental biology and regenerative medicine due to its critical role in various signaling pathways that govern embryonic development, cell differentiation, and tissue patterning. Shh is a member of the Hedgehog signaling pathway, which is essential for proper organogenesis and is implicated in the regulation of cell proliferation and fate determination during embryonic development. Abnormal Shh signaling has been linked to a variety of congenital disorders and cancers, making it a crucial target for therapeutic interventions. Research focuses on producing Shh recombinant proteins to better understand their structure-function relationships and to explore their potential applications in tissue engineering and regenerative therapies. For instance, Shh has shown promise in promoting neural stem cell proliferation and differentiation, indicating its potential for treating neurodegenerative diseases or spinal cord injuries. Moreover, studying Shh in a controlled recombinant form allows researchers to investigate its interactions with other signaling molecules and its downstream effects on gene expression. Overall, the exploration of Shh recombinant protein holds significant promise for advancing our understanding of developmental processes and for developing innovative therapeutic strategies in various medical fields.
