Analytical Data
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Gene name
KCNJ11
- Application
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Alternative Names
KCNJ11; ATP-sensitive inward rectifier potassium channel 11; IKATP; Inward rectifier K(+ channel Kir6.2; Potassium channel. inwardly rectifying subfamily J member 11
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q14654
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Expression Region
1-390aa
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AA Sequence
MLSRKGIIPEEYVLTRLAEDPAKPRYRARQRRARFVSKKGNCNVAHKNIREQGRFLQDVFTTLVDLKWPHTLLIFTMSFLCSWLLFAMAWWLIAFAHGDLAPSEGTAEPCVTSIHSFSSAFLFSIEVQVTIGFGGRMVTEECPLAILILIVQNIVGLMINAIMLGCIFMKTAQAHRRAETLIFSKHAVIALRHGRLCFMLRVGDLRKSMIISATIHMQVVRKTTSPEGEVVPLHQVDIPMENGVGGNSIFLVAPLIIYHVIDANSPLYDLAPSDLHHHQDLEIIVILEGVVETTGITTQARTSYLADEILWGQRFVPIVAEEDGRYSVDYSKFGNTVKVPTPLCTARQLDEDHSLLEALTLASARGPLRKRSVPMAKAKPKFSISPDSLS
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Molecular Weight
69.9 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
KCNJ11, a member of the inward rectifier potassium channel family, encodes the Kir6.2 protein, which plays a crucial role in regulating insulin secretion in pancreatic beta cells. Mutations in KCNJ11 can lead to congenital hyperinsulinism or neonatal diabetes, highlighting its significance in glucose homeostasis. The protein interacts with sulfonylurea receptors (SUR1) to form the ATP-sensitive potassium (KATP) channel, a key player in linking cellular metabolic signals to insulin release. Research on recombinant KCNJ11 protein has advanced our understanding of its structure-function relationship, electrophysiological properties, and pharmacological profiles. The ability to produce and manipulate recombinant KCNJ11 has paved the way for detailed biochemical studies, providing insights into the activation and inhibition mechanisms of KATP channels and their roles in various pathophysiological conditions. Understanding the function of KCNJ11 is not only crucial for elucidating the molecular basis of related metabolic disorders but also for developing targeted therapies that can modulate its activity. Thus, the study of KCNJ11 recombinant protein is significant for both basic science and clinical applications, as it holds potential for novel interventions in diabetes management and other related diseases.
