Analytical Data
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基因名
KCNJ11
- Application
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别名
KCNJ11; ATP-sensitive inward rectifier potassium channel 11; IKATP; Inward rectifier K(+ channel Kir6.2; Potassium channel. inwardly rectifying subfamily J member 11
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q14654
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表达区间
1-390aa
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氨基酸序列
MLSRKGIIPEEYVLTRLAEDPAKPRYRARQRRARFVSKKGNCNVAHKNIREQGRFLQDVFTTLVDLKWPHTLLIFTMSFLCSWLLFAMAWWLIAFAHGDLAPSEGTAEPCVTSIHSFSSAFLFSIEVQVTIGFGGRMVTEECPLAILILIVQNIVGLMINAIMLGCIFMKTAQAHRRAETLIFSKHAVIALRHGRLCFMLRVGDLRKSMIISATIHMQVVRKTTSPEGEVVPLHQVDIPMENGVGGNSIFLVAPLIIYHVIDANSPLYDLAPSDLHHHQDLEIIVILEGVVETTGITTQARTSYLADEILWGQRFVPIVAEEDGRYSVDYSKFGNTVKVPTPLCTARQLDEDHSLLEALTLASARGPLRKRSVPMAKAKPKFSISPDSLS
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分子量
69.9 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
KCNJ11, a member of the inward rectifier potassium channel family, encodes the Kir6.2 protein, which plays a crucial role in regulating insulin secretion in pancreatic beta cells. Mutations in KCNJ11 can lead to congenital hyperinsulinism or neonatal diabetes, highlighting its significance in glucose homeostasis. The protein interacts with sulfonylurea receptors (SUR1) to form the ATP-sensitive potassium (KATP) channel, a key player in linking cellular metabolic signals to insulin release. Research on recombinant KCNJ11 protein has advanced our understanding of its structure-function relationship, electrophysiological properties, and pharmacological profiles. The ability to produce and manipulate recombinant KCNJ11 has paved the way for detailed biochemical studies, providing insights into the activation and inhibition mechanisms of KATP channels and their roles in various pathophysiological conditions. Understanding the function of KCNJ11 is not only crucial for elucidating the molecular basis of related metabolic disorders but also for developing targeted therapies that can modulate its activity. Thus, the study of KCNJ11 recombinant protein is significant for both basic science and clinical applications, as it holds potential for novel interventions in diabetes management and other related diseases.
