Analytical Data
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基因名
UROD
- Application
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别名
UROD；Uroporphyrinogen decarboxylase
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P06132
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表达区间
1-367aa
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氨基酸序列
MGSSHHHHHH SSGLVPRGSH MEANGLGPQG FPELKNDTFL RAAWGEETDY TPVWCMRQAG RYLPEFRETR AAQDFFSTCR SPEACCELTL QPLRRFPLDA AIIFSDILVV PQALGMEVTM VPGKGPSFPE PLREEQDLER LRDPEVVASE LGYVFQAITL TRQRLAGRVP LIGFAGAPWT LMTYMVEGGG SSTMAQAKRW LYQRPQASHQ LLRILTDALV PYLVGQVVAG AQALQLFESH AGHLGPQLFN KFALPYIRDV AKQVKARLRE AGLAPVPMII FAKDGHFALE ELAQAGYEVV GLDWTVAPKK ARECVGKTVT LQVNLDPCAL YASEEEIGQL VKQMLDDFGP HRYIANLGHG LYPDMDPEHV GAFVDAVHKH SRLLRQN
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分子量
43 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
UROD (Uroporphyrinogen Decarboxylase) is a key enzyme in the heme biosynthesis pathway, responsible for converting uroporphyrinogen to coproporphyrinogen, a crucial step in the production of heme. Deficiency in UROD activity is associated with a rare genetic disorder known as porphyria cutanea tarda (PCT), which leads to the accumulation of porphyrins and manifests in skin lesions and photosensitivity. Research into UROD recombinant protein aims to better understand its structure, function, and regulatory mechanisms, as well as to develop potential therapeutic strategies for managing PCT and other related disorders. Advances in recombinant DNA technology have allowed for the expression and purification of UROD, enabling detailed biochemical characterization and studies on its enzymatic activity, stability, and interactions with other biomolecules. This research not only enhances our understanding of porphyrin metabolism but also opens avenues for novel therapeutic interventions, including enzyme replacement therapy and pharmacological modulation of UROD activity. By elucidating the molecular basis of UROD function and its role in porphyrin disorders, scientists aspire to develop targeted treatments that could significantly improve the quality of life for affected individuals. Additionally, understanding UROD's structure-function relationship is critical for the design of inhibitors that could be used in other pathological contexts where heme synthesis is dysregulated, underscoring its relevance in both basic and applied biomedical research.
