Analytical Data
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基因名
IL-8
- Application
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别名
CXCL8;IL8;Interleukin-8
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P10145
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表达区间
28-99aa
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氨基酸序列
SAKELRCQCI KTYSKPFHPK FIKELRVIES GPHCANTEII VKLSDGRELC LDPKENWVQR VVEKFLKRAE NS
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分子量
8.4 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Interleukin-8 (IL-8), a pro-inflammatory cytokine, plays a critical role in the immune response by promoting the recruitment and activation of neutrophils to sites of infection and inflammation. Initially identified as a potent chemokine in the inflammatory cascade, IL-8 is produced by various cell types, including macrophages, epithelial cells, and endothelial cells, in response to inflammatory stimuli. Its dysregulation has been implicated in numerous pathological conditions, such as chronic inflammatory diseases, autoimmune disorders, and cancer progression. The advent of recombinant DNA technology has enabled the production of IL-8 in a purified form, facilitating in-depth studies into its biological functions and therapeutic potential. Research on IL-8 recombinant protein has expanded to explore its role in modulating immune responses, the mechanisms governing its signaling pathways, and its potential applications in clinical settings, such as targeted therapies for inflammation-related diseases. Furthermore, understanding the structure-function relationships of IL-8 has opened avenues for developing IL-8 antagonists, aimed at mitigating its pathological effects. As the interest in cytokine biology continues to grow, IL-8 remains a focal point for developing novel therapeutic strategies to harness the immune response effectively while minimizing the risks associated with excessive inflammation.












