Analytical Data
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Gene name
JMJD3
- Application
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Alternative Names
Histone demethylase JMJD3; JmjC domain containing protein 3; JmjC domain-containing protein 3; Jumonji D3; Jumonji domain containing 3; Jumonji domain containing 3 histone lysine demethylase; Jumonji domain containing protein 3; Jumonji domain-containing protein 3; Kdm6b; KDM6B_HUMAN; KIAA0346; Lysine demethylase 6B
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O15054
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Expression Region
1043-1643aa
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AA Sequence
KAKPPTAP APPSAPAPSA QPTPPSASVP GKKAREEAPG PPGVSRADML KLRSLSEGPP KELKIRLIKV ESGDKETFIA SEVEERRLRM ADLTISHCAA DVVRASRNAK VKGKFRESYL SPAQSVKPKI NTEEKLPREK LNPPTPSIYL ESKRDAFSPV LLQFCTDPRN PITVIRGLAG SLRLNLGLFS TKTLVEASGE HTVEVRTQVQ QPSDENWDLT GTRQIWPCES SRSHTTIAKY AQYQASSFQE SLQEEKESED EESEEPDSTT GTPPSSAPDP KNHHIIKFGT NIDLSDAKRW KPQLQELLKL PAFMRVTSTG NMLSHVGHTI LGMNTVQLYM KVPGSRTPGH QENNNFCSVN INIGPGDCEW FAVHEHYWET ISAFCDRHGV DYLTGSWWPI LDDLYASNIP VYRFVQRPGD LVWINAGTVH WVQATGWCNN IAWNVGPLTA YQYQLALERY EWNEVKNVKS IVPMIHVSWN VARTVKISDP DLFKMIKFCL LQSMKHCQVQ RESLVRAGKK IAYQGRVKDE PAYYCNECDV EVFNILFVTS ENGSRNTYLV HCEGCARRRS AGLQGVVVLE QYRTEELAQA YDAFTLVRAR RARGQRRRAL GQAAGTGFGS PAAPFPEPPP AFSPQAPAST SR
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Molecular Weight
71 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
JMJD3 (Jumonji domain-containing protein 3) is a member of the Jumonji C (JmjC) domain-containing demethylases, which play crucial roles in epigenetic regulation by removing methyl groups from histones, thereby influencing gene expression. The research on JMJD3 has gained significant attention due to its involvement in various biological processes, including development, differentiation, and response to environmental changes. Dysregulation of JMJD3 has been implicated in several diseases, particularly cancer, where it can modulate the tumor microenvironment and influence tumor progression. Moreover, JMJD3 has been identified as a key factor in the regulation of immune responses, linking it to inflammatory diseases and autoimmune disorders. The study of recombinant JMJD3 protein is essential for understanding its biochemical properties, active sites, and interaction with other cellular components. It serves as a valuable tool for elucidating the molecular mechanisms underlying its functions and for exploring potential therapeutic targets in related pathologies. Through structural and functional analyses, researchers aim to uncover how JMJD3 mediates epigenetic modifications and its broader implications in health and disease. As the understanding of JMJD3 continues to evolve, the development of specific inhibitors or modulators could pave the way for novel treatment strategies in cancer and other disorders associated with aberrant JMJD3 activity.
