Analytical Data
-
Gene name
ITPKA
- Application
-
Alternative Names
ITPKA; Inositol-trisphosphate 3-kinase A; EC 2.7.1.127; Inositol 1.4.5-trisphosphate 3-kinase A; IP3 3-kinase A; IP3K A; InsP 3-kinase A
-
Species
Human
-
Source
E. coli
-
Tag
His tag N-Terminus
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
P23677
-
Expression Region
1-461aa
-
AA Sequence
MTLPGGPTGM ARPGGARPCS PGLERAPRRS VGELRLLFEA RCAAVAAAAA AGEPRARGAK RRGGQVPNGL PRAPPAPVIP QLTVTAEEPD VPPTSPGPPE RERDCLPAAG SSHLQQPRRL STSSVSSTGS SSLLEDSEDD LLSDSESRSR GNVQLEAGED VGQKNHWQKI RTMVNLPVIS PFKKRYAWVQ LAGHTGSFKA AGTSGLILKR CSEPERYCLA RLMADALRGC VPAFHGVVER DGESYLQLQD LLDGFDGPCV LDCKMGVRTY LEEELTKARE RPKLRKDMYK KMLAVDPEAP TEEEHAQRAV TKPRYMQWRE GISSSTTLGF RIEGIKKADG SCSTDFKTTR SREQVLRVFE EFVQGDEEVL RRYLNRLQQI RDTLEVSEFF RRHEVIGSSL LFVHDHCHRA GVWLIDFGKT TPLPDGQILD HRRPWEEGNR EDGYLLGLDN LIGILASLAE R
-
Molecular Weight
51 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
ITPKA (Inositol Trisphosphate Kinase A) is a member of the inositol phosphate kinase family, playing a crucial role in signal transduction pathways that regulate various cellular processes, including growth, differentiation, and apoptosis. The study of ITPKA is important because it influences intracellular calcium signaling and impacts several physiological functions in neurons and other cell types. Dysregulation of ITPKA has been implicated in various diseases, including cancer and neurodegenerative disorders. In recent years, researchers have focused on the expression and purification of recombinant ITPKA to better understand its biochemical properties and functional mechanisms. The generation of recombinant ITPKA not only allows for detailed kinetic studies but also facilitates the exploration of drug design possibilities targeting its enzymatic activity. By utilizing techniques such as site-directed mutagenesis, scientists can investigate the effects of specific amino acid changes on the enzyme's function and regulation. Advances in protein expression systems, such as bacterial and eukaryotic platforms, have improved the yield and activity of the purified ITPKA, enabling more sophisticated experimental approaches. These studies contribute valuable insights into the role of ITPKA in health and disease, highlighting its potential as a therapeutic target. Understanding the structural and functional dynamics of ITPKA will pave the way for novel interventions in related disorders, making it an integral focus of ongoing research in molecular and cellular biology.
