Analytical Data
-
Gene name
HSD17b3
- Application
-
Alternative Names
HSD17b3；EDH17B3；SDR12C2；17-beta-hydroxysteroid dehydrogenase type 3
-
Species
Human
-
Source
E. coli
-
Tag
His tag N-Terminus
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
P37058
-
Expression Region
1-310aa
-
AA Sequence
MGDVLEQFFI LTGLLVCLAC LAKCVRFSRC VLLNYWKVLP KSFLRSMGQW AVITGAGDGI GKAYSFELAK RGLNVVLISR TLEKLEAIAT EIERTTGRSV KIIQADFTKD DIYEHIKEKL AGLEIGILVN NVGMLPNLLP SHFLNAPDEI QSLIHCNITS VVKMTQLILK HMESRQKGLI LNISSGIALF PWPLYSMYSA SKAFVCAFSK ALQEEYKAKE VIIQVLTPYA VSTAMTKYLN TNVITKTADE FVKESLNYVT IGGETCGCLA HEILAGFLSL IPAWAFYSGA FQRLLLTHYV AYLKLNTKVR
-
Molecular Weight
34.5 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
HSD17B3, or 17beta-hydroxysteroid dehydrogenase type 3, is an enzyme that plays a critical role in steroid hormone biosynthesis, particularly in the conversion of androstenedione to testosterone, which is essential for male sexual differentiation and reproduction. Mutations in the HSD17B3 gene can lead to disorders of sexual development (DSDs) in males, characterized by underdeveloped genitalia and other reproductive anomalies. The study of recombinant HSD17B3 proteins has gained significance in understanding these pathological conditions and developing potential therapeutic strategies. By producing and purifying recombinant HSD17B3, researchers can investigate the enzyme's structure-function relationship, its catalytic mechanisms, and how specific mutations affect its activity. This research not only contributes to a deeper understanding of steroidogenesis but also aids in the identification of biomarkers for DSDs and informs clinical approaches for diagnosis and treatment. Additionally, recombinant HSD17B3 proteins hold promise for drug development that targets hormonal imbalances or provides insights into novel contraceptive methods. Understanding the function and regulation of HSD17B3 through recombinant studies is crucial for advancing both basic and clinical endocrinology.
