Analytical Data
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基因名
KPNa2
- Application
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别名
KPNa2；RCH1；SRP1；Importin subunit alpha-1
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P52292
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表达区间
1-529aa
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氨基酸序列
MGSSHHHHHH SSGLVPRGSH MGSHMSTNEN ANTPAARLHR FKNKGKDSTE MRRRRIEVNV ELRKAKKDDQ MLKRRNVSSF PDDATSPLQE NRNNQGTVNW SVDDIVKGIN SSNVENQLQA TQAARKLLSR EKQPPIDNII RAGLIPKFVS FLGRTDCSPI QFESAWALTN IASGTSEQTK VVVDGGAIPA FISLLASPHA HISEQAVWAL GNIAGDGSVF RDLVIKYGAV DPLLALLAVP DMSSLACGYL RNLTWTLSNL CRNKNPAPPI DAVEQILPTL VRLLHHDDPE VLADTCWAIS YLTDGPNERI GMVVKTGVVP QLVKLLGASE LPIVTPALRA IGNIVTGTDE QTQVVIDAGA LAVFPSLLTN PKTNIQKEAT WTMSNITAGR QDQIQQVVNH GLVPFLVSVL SKADFKTQKE AVWAVTNYTS GGTVEQIVYL VHCGIIEPLM NLLTAKDTKI ILVILDAISN IFQAAENLGE TEKLSIMIEE CGGLDKIEAL QNHENESVYK ASLSLIEKYF SVEEEEDQNV VPETTSEGYT FQVQDGAPGT FNF
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分子量
61 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
KPNa2 is a member of the karyopherin family, which plays a crucial role in the nuclear transport of macromolecules. This protein functions as a nuclear import receptor, facilitating the translocation of various substrates, including proteins with nuclear localization signals, across the nuclear pore complex. The study of KPNa2 has gained attention due to its implications in cellular processes, such as gene expression, signal transduction, and cell cycle regulation. Abnormalities in nuclear transport mechanisms, including those involving KPNa2, can contribute to various diseases, including cancer and viral infections, highlighting the importance of understanding its structure and function. Recent research efforts have been focused on the characterization of KPNa2 at both structural and functional levels, employing techniques such as X-ray crystallography, NMR spectroscopy, and biochemical assays. These studies aim to elucidate the detailed mechanisms of KPNa2-mediated nuclear transport and its interactions with cargo proteins. Furthermore, the exploration of KPNa2's role in disease states could provide valuable insights for therapeutic strategies targeting nuclear transport pathways. By examining the reconstitution and function of KPNa2, researchers hope to develop a comprehensive understanding of its biological significance and therapeutic potential, paving the way for novel interventions in conditions arising from dysregulated nuclear transport.
