Cat: PA2000-4355

Recombinant Human D266N Protein,His

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Analytical Data

  • Gene name

    D266N

  • Application

    SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

  • Alternative Names

    D266N;

  • Species

    Human

  • Source

    E. coli

  • Tag

    His tag N-Terminus

  • Purity

    Greater than 90% as determined by SDS-PAGE.

  • Uniprot

    P30530

  • Expression Region

    26-451aa

  • AA Sequence

    APRGTQAEESPFVGNPGNITGARGLTGTLRCQLQVQGEPPEVHWLRDGQILELADSTQTQVPLGEDEQDDWIVVSQLRITSLQLSDTGQYQCLVFLGHQTFVSQPGYVGLEGLPYFLEEPEDRTVAANTPFNLSCQAQGPPEPVDLLWLQDAVPLATAPGHGPQRSLHVPGLNKTSSFSCEAHNAKGVTTSRTATITVLPQQPRNLHLVSRQPTELEVAWTPGLSGIYPLTHCTLQAVLSNDGMGIQAGEPDPPEEPLTSQASVPPHQLRLGSLHPHTPYHIRVACTSSQGPSSWTHWLPVETPEGVPLGPPENISATRNGSQAFVHWQEPRAPLQGTLLGYRLAYQGQDTPEVLMDIGLRQEVTLELQGDGSVSNLTVCVAAYTAAGDGPWSLPVPLEAWRPGQAQPVHQLVKEPSTPAFSWPWW

  • Molecular Weight

    75.1 kDa

  • Endotoxin

    < 1.0 EU per μg protein as determined by the LAL method.

  • Form

    Freeze-dried powder

  • Buffer formulation

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • Reconstitution

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • Customization

    Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

  • Stability Test

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • Storage & Shelf Life

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • Shipping

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

Quality inspection process

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Protein Description

The D266N recombinant protein has garnered attention in the fields of molecular biology and biochemistry due to its potential implications in understanding disease mechanisms and therapeutic development. This protein variant is a specific mutation in the gene encoding for a target enzyme or receptor, which is often linked to various pathological conditions. Research into the D266N mutation aims to unravel its effects on protein structure, function, and stability, as mutations in critical residues can lead to altered enzymatic activity or disrupted interactions with other biomolecules. Such studies are crucial for elucidating the biological roles of this protein and its involvement in metabolic pathways or signaling cascades. Additionally, the recombinant expression of D266N allows for the investigation of its properties in vitro, enabling researchers to assess how this specific alteration can influence drug binding or the efficacy of therapeutic agents. Understanding the functional consequences of the D266N mutation can contribute to the development of targeted treatments, personalized medicine approaches, and the discovery of biomarkers for disease diagnosis and prognosis. Furthermore, the study of this recombinant protein can provide insights into broader biological processes, highlighting the importance of protein engineering and molecular characterization in modern biomedical research. As the search for innovative solutions to complex diseases continues, the D266N recombinant protein serves as a valuable model for exploring the intricate relationships between genetic variations and pathophysiological outcomes.

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