Analytical Data
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基因名
IDH3A
- Application
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别名
H IDH alpha; HIDH alpha; IDH3A; IDH3A_HUMAN; Isocitrate dehydrogenase (NAD+) alpha chain precursor; Isocitrate dehydrogenase [NAD] subunit alpha; Isocitrate dehydrogenase [NAD] subunit alpha mitochondrial; Isocitrate dehydrogenase 3 (NAD+) alpha; Isocitrate dehydrogenase; Isocitric dehydrogenase; Isocitric dehydrogenase subunit alpha; mitochondrial; NAD
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P50213
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表达区间
1-366aa
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氨基酸序列
MAGPAWISKVSRLLGAFHNPKQVTRGFTGGVQTVTLIPGDGIGPEISAAVMKIFDAAKAPIQWEERNVTAIQGPGGKWMIPSEAKESMDKNKMGLKGPLKTPIAAGHPSMNLLLRKTFDLYANVRPCVSIEGYKTPYTDVNIVTIRENTEGEYSGIEHVIVDGVVQSIKLITEGASKRIAEFAFEYARNNHRSNVTAVHKANIMRMSDGLFLQKCREVAESCKDIKFNEMYLDTVCLNMVQDPSQFDVLVMPNLYGDILSDLCAGLIGGLGVTPSGNIGANGVAIFESVHGTAPDIAGKDMANPTALLLSAVMMLRHMGLFDHAARIEAACFATIKDGKSLTKDLGGNAKCSDFTEEICRRVKDLD
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分子量
66 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
IDH3A (Isocitrate Dehydrogenase 3 Alpha) is a crucial enzyme involved in the tricarboxylic acid (TCA) cycle, playing a significant role in cellular respiration and energy production in mammals. As a key component of the mitochondrial IDH complex, IDH3A catalyzes the oxidative decarboxylation of isocitrate to α-ketoglutarate, and its activity is tightly regulated by the cellular energy status, which makes it essential for maintaining metabolic homeostasis. Aberrant regulation or mutations in IDH3A have been linked to various metabolic disorders and pathologies, including cancer, where alterations in the TCA cycle can promote tumorigenesis by enabling cancer cells to adapt to hypoxic environments and utilize alternative metabolic pathways. Recent research has focused on the structural and functional characterization of recombinant IDH3A, enabling scientists to investigate its enzymatic mechanisms, identify potential inhibitors, and explore the therapeutic implications of targeting IDH enzymes. Advancements in protein engineering and structural biology techniques, such as X-ray crystallography and cryo-electron microscopy, have provided invaluable insights into the conformational dynamics of IDH3A, facilitating the development of novel metabolic modulators for cancer therapy. Understanding the regulatory mechanisms and metabolic role of IDH3A in cellular contexts not only enhances our comprehension of basic metabolic processes but also opens avenues for innovative strategies in targeted cancer treatments and metabolic disease management. As the field continues to evolve, further studies on recombinant IDH3A may lead to significant breakthroughs in both fundamental biochemistry and clinical applications.
