Analytical Data
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基因名
TGM1
- Application
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别名
TGM1；KTG；Protein-glutamine gamma-glutamyltransferase K
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P22735
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表达区间
41-510aa
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氨基酸序列
RSFWARCCGCCSCRNAADDDWGPEPSDSRGRGSSSGTRRPGSRGSDSRRPVSRGSGVNAAGDGTIREGMLVVNGVDLLSSRSDQNRREHHTDEYEYDELIVRRGQPFHMLLLLSRTYESSDRITLELLIGNNPEVGKGTHVIIPVGKGGSGGWKAQVVKASGQNLNLRVHTSPNAIIGKFQFTVRTQSDAGEFQLPFDPRNEIYILFNPWCPEDIVYVDHEDWRQEYVLNESGRIYYGTEAQIGERTWNYGQFDHGVLDACLYILDRRGMPYGGRGDPVNVSRVISAMVNSLDDNGVLIGNWSGDYSRGTNPSAWVGSVEILLSYLRTGYSVPYGQCWVFAGVTTTVLRCLGLATRTVTNFNSAHDTDTSLTMDIYFDENMKPLEHLNHDSVWNFHVWNDCWMKRPDLPSGFDGWQVVDATPQETSSGIFCCGPCSVESIKNGLVYMKYDTPFIFAEVNSDKVYWQRQDD
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分子量
59.6 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
TGM1, or Transglutaminase 1, is a crucial enzyme involved in the formation of the outermost layer of the skin, playing a significant role in keratinocyte differentiation and barrier function. Mutations in the TGM1 gene are linked to autosomal recessive ichthyosis, a genetic skin disorder characterized by dry, scaly skin due to impaired barrier formation. Recent research has emphasized the importance of TGM1 in not just skin integrity but also its potential involvement in other physiological pathways, such as apoptosis and cell signaling. Recombinant TGM1 protein has become a vital tool for studying these functions, offering insights into enzyme kinetics, substrate interactions, and the molecular mechanisms underlying skin diseases. By producing TGM1 in a recombinant system, researchers can obtain large quantities of the protein for in vitro studies, enabling them to explore therapeutic options and validate the enzyme's role in various biological processes. Furthermore, understanding the structure-function relationship of TGM1 can aid in the design of novel treatments for skin disorders associated with its dysfunction, paving the way for innovative strategies in dermatology. Overall, the study of recombinant TGM1 protein not only holds promise for elucidating its fundamental biological roles but also for developing targeted therapies for related skin conditions.
