Analytical Data
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基因名
HADHB
- Application
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别名
HADHB;Trifunctional enzyme subunit beta. mitochondrial
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P55084
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表达区间
34-474aa
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氨基酸序列
MGSSHHHHHH SSGLVPRGSH MGSAAPAVQTKTKKTLAKPNIRNVVVVDGVRTPFLLSGTSYKDLMPHDLA RAALTGLLHRTSVPKEVVDYIIFGTVIQEVKTSNVAREAALGAGFSDKTP AHTVTMACISANQAMTTGVGLIASGQCDVIVAGGVELMSDVPIRHSRKMR KLMLDLNKAKSMGQRLSLISKFRFNFLAPELPAVSEFSTSETMGHSADRL AAAFAVSRLEQDEYALRSHSLAKKAQDEGLLSDVVPFKVPGKDTVTKDNG IRPSSLEQMAKLKPAFIKPYGTVTAANSSFLTDGASAMLIMAEEKALAMG YKPKAYLRDFMYVSQDPKDQLLLGPTYATPKVLEKAGLTMNDIDAFEFHE AFSGQILANFKAMDSDWFAENYMGRKTKVGLPPLEKFNNWGGSLSLGHPF GATGCRLVMAAANRLRKEGGQYGLVAACAAGGQGHAMIVEAYPK
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分子量
50 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
HADHB (hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit beta) is a crucial enzyme involved in the mitochondrial fatty acid oxidation pathway, specifically in the catabolism of long-chain fatty acids. Mutations in the HADHB gene lead to a rare but severe metabolic disorder known as mitochondrial trifunctional protein deficiency, characterized by hypoketotic hypoglycemia, muscle weakness, and increased accumulation of toxic fatty acid metabolites. The significance of HADHB extends beyond basic biochemistry; it has implications for understanding metabolic syndromes and developing potential therapeutic strategies. Researchers aim to elucidate the structural and functional characteristics of HADHB through recombinant protein techniques, which involve producing the protein in a host system, often Escherichia coli or yeast. Understanding the enzyme's activity, kinetics, and interactions with other metabolic pathways can provide insights into its role in cellular energy homeostasis and potential links to more common conditions such as obesity and type 2 diabetes. Moreover, recombinant HADHB may serve as a valuable tool for drug discovery and the assessment of therapeutic compounds aimed at correcting the enzyme's deficiencies. As such, studies of HADHB recombinant protein are integral not only to the basic understanding of mitochondrial function and energy metabolism but also to the broader landscape of metabolic disease research.
