Analytical Data
-
Gene name
HS3ST3A1
- Application
-
Alternative Names
HS3ST3A1; 3OST3A1; HS3ST3A; UNQ2551/PRO6180Heparan sulfate glucosamine 3-O-sulfotransferase 3A1; EC 2.8.2.30; Heparan sulfate D-glucosaminyl 3-O-sulfotransferase 3A1; 3-OST-3A; Heparan sulfate 3-O-sulfotransferase 3A1; h3-OST-3A
-
Species
Human
-
Source
E. coli
-
Tag
GST-tag at N-terminal
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
Q9Y663
-
Expression Region
1-406aa
-
AA Sequence
MAPPGPASALSTSAEPLSRSIFRKFLLMLCSLLTSLYVFYCLAERCQTLSGPVVGLSGGGEEAGAPGGGVLAGGPRELAVWPAAAQRKRLLQLPQWRRRRPPAPRDDGEEAAWEEESPGLSGGPGGSGAGSTVAEAPPGTLALLLDEGSKQLPQAIIIGVKKGGTRALLEFLRVHPDVRAVGAEPHFFDRSYDKGLAWYRDLMPRTLDGQITMEKTPSYFVTREAPARISAMSKDTKLIVVVRDPVTRAISDYTQTLSKRPDIPTFESLTFKNRTAGLIDTSWSAIQIGIYAKHLEHWLRHFPIRQMLFVSGERLISDPAGELGRVQDFLGLKRIITDKHFYFNKTKGFPCLKKAEGSSRPHCLGKTKGRTHPEIDREVVRRLREFYRPFNLKFYQMTGHDFGWDG
-
Molecular Weight
71.3 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
HS3ST3A1 (Heparan Sulfate 3-O-Sulfotransferase 3A1) is an enzyme involved in the biosynthesis of heparan sulfate, a glycosaminoglycan that plays a crucial role in cell signaling, development, and tissue homeostasis. The modification of heparan sulfate through sulfation is essential for its function, impacting various biological processes including cell adhesion, growth factor signaling, and coagulation. Dysregulation of HS3ST3A1 has been implicated in several diseases, such as cancer and developmental disorders. Its ability to modify the structure of heparan sulfate chains influences their interaction with ligands like growth factors, morphogens, and extracellular matrix components. Given its significance in cell-matrix interactions and signaling pathways, the recombinant expression and characterization of HS3ST3A1 are vital for understanding its functional roles and potential therapeutic applications. Furthermore, the study of HS3ST3A1 may provide insights into the mechanisms of heparan sulfate-related pathologies, thereby opening avenues for the development of targeted strategies for disease intervention. Consequently, research on this recombinant protein not only contributes to basic biology but also has implications for clinical applications, emphasizing the need for deeper exploration of its structure and function.
