Analytical Data
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基因名
HS3ST3A1
- Application
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别名
HS3ST3A1; 3OST3A1; HS3ST3A; UNQ2551/PRO6180Heparan sulfate glucosamine 3-O-sulfotransferase 3A1; EC 2.8.2.30; Heparan sulfate D-glucosaminyl 3-O-sulfotransferase 3A1; 3-OST-3A; Heparan sulfate 3-O-sulfotransferase 3A1; h3-OST-3A
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9Y663
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表达区间
1-406aa
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氨基酸序列
MAPPGPASALSTSAEPLSRSIFRKFLLMLCSLLTSLYVFYCLAERCQTLSGPVVGLSGGGEEAGAPGGGVLAGGPRELAVWPAAAQRKRLLQLPQWRRRRPPAPRDDGEEAAWEEESPGLSGGPGGSGAGSTVAEAPPGTLALLLDEGSKQLPQAIIIGVKKGGTRALLEFLRVHPDVRAVGAEPHFFDRSYDKGLAWYRDLMPRTLDGQITMEKTPSYFVTREAPARISAMSKDTKLIVVVRDPVTRAISDYTQTLSKRPDIPTFESLTFKNRTAGLIDTSWSAIQIGIYAKHLEHWLRHFPIRQMLFVSGERLISDPAGELGRVQDFLGLKRIITDKHFYFNKTKGFPCLKKAEGSSRPHCLGKTKGRTHPEIDREVVRRLREFYRPFNLKFYQMTGHDFGWDG
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分子量
71.3 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
HS3ST3A1 (Heparan Sulfate 3-O-Sulfotransferase 3A1) is an enzyme involved in the biosynthesis of heparan sulfate, a glycosaminoglycan that plays a crucial role in cell signaling, development, and tissue homeostasis. The modification of heparan sulfate through sulfation is essential for its function, impacting various biological processes including cell adhesion, growth factor signaling, and coagulation. Dysregulation of HS3ST3A1 has been implicated in several diseases, such as cancer and developmental disorders. Its ability to modify the structure of heparan sulfate chains influences their interaction with ligands like growth factors, morphogens, and extracellular matrix components. Given its significance in cell-matrix interactions and signaling pathways, the recombinant expression and characterization of HS3ST3A1 are vital for understanding its functional roles and potential therapeutic applications. Furthermore, the study of HS3ST3A1 may provide insights into the mechanisms of heparan sulfate-related pathologies, thereby opening avenues for the development of targeted strategies for disease intervention. Consequently, research on this recombinant protein not only contributes to basic biology but also has implications for clinical applications, emphasizing the need for deeper exploration of its structure and function.
