Analytical Data
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Gene name
DCT
- Application
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Alternative Names
DCT；TYRP2；L-dopachrome tautomerase
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P40126
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Expression Region
24-472aa
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AA Sequence
QFPRVCMTVDSLVNKECCPRLGAESANVCGSQQGRGQCTEVRADTRPWSGPYILRNQDDRELWPRKFFHRTCKCTGNFAGYNCGDCKFGWTGPNCERKKPPVIRQNIHSLSPQEREQFLGALDLAKKRVHPDYVITTQHWLGLLGPNGTQPQFANCSVYDFFVWLHYYSVRDTLLGPGRPYRAIDFSHQGPAFVTWHRYHLLCLERDLQRLIGNESFALPYWNFATGRNECDVCTDQLFGAARPDDPTLISRNSRFSSWETVCDSLDDYNHLVTLCNGTYEGLLRRNQMGRNSMKLPTLKDIRDCLSLQKFDNPPFFQNSTFSFRNALEGFDKADGTLDSQVMSLHNLVHSFLNGTNALPHSAANDPIFVVLHSFTDAIFDEWMKRFNPPADAWPQELAPIGHNRMYNMVPFFPPVTNEELFLTSDQLGYSYAIDLPVSVEETPGWPTT
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Molecular Weight
71.3 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
DCT (Dopachrome tautomerase) is an enzyme that plays a significant role in the melanogenesis pathway, which is responsible for melanin production in the body. Understanding DCT and its function has vital implications in dermatology, particularly concerning skin pigmentation disorders and melanoma, a type of skin cancer. Research on recombinant DCT proteins has gained traction as it allows for detailed studies of the enzyme's structure, function, and interactions within biological systems. By producing DCT through recombinant DNA technology, scientists can generate large quantities of the protein, facilitating high-resolution structural analyses and functional assays that might be difficult to achieve with native proteins. Furthermore, studying recombinant DCT enables the exploration of its potential as a therapeutic target and biomarker for melanocytic diseases. This line of research not only contributes to our understanding of pigment biology but also opens avenues for developing novel treatments for skin disorders linked to altered melanin production. As we delve deeper into the molecular mechanisms regulating DCT activity and expression, we gain valuable insights that could lead to innovative strategies for managing pigmentation conditions and improving skin health.
