Cat: PA2000-80DB

Recombinant Human GSTa3 Protein,His

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Analytical Data

  • Gene name

    GSTa3

  • Application

    SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

  • Alternative Names

    GSTa3;Glutathione S-transferase A3

  • Species

    Human

  • Source

    E. coli

  • Tag

    His tag N-Terminus

  • Purity

    Greater than 90% as determined by SDS-PAGE.

  • Uniprot

    Q16772

  • Expression Region

    2-222aa

  • AA Sequence

    AGKPKLHYF NGRGRMEPIR WLLAAAGVEF EEKFIGSAED LGKLRNDGSL MFQQVPMVEI DGMKLVQTRA ILNYIASKYN LYGKDIKERA LIDMYTEGMA DLNEMILLLP LCRPEEKDAK IALIKEKTKS RYFPAFEKVL QSHGQDYLVG NKLSRADISL VELLYYVEEL DSSLISNFPL LKALKTRISN LPTVKKFLQP GSPRKPPADA KALEEARKIF RF

  • Endotoxin

    < 1.0 EU per μg protein as determined by the LAL method.

  • Form

    Freeze-dried powder

  • Buffer formulation

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • Reconstitution

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • Customization

    Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

  • Stability Test

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • Storage & Shelf Life

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • Shipping

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

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Protein Description

GSTa3 is a glutathione S-transferase (GST) from the alpha class, which plays a pivotal role in detoxifying harmful compounds and is involved in various cellular processes. The study of GSTa3 has garnered attention due to its significant implications in pharmacology, particularly in drug metabolism and resistance mechanisms in cancer cells. Research indicates that GSTa3 is overexpressed in various tumors, suggesting a potential role in tumor progression and chemoresistance. Understanding the structure and function of GSTa3 through recombinant protein studies can provide insights into its catalytic mechanisms and interactions with substrates. Additionally, GSTa3 serves as a useful model for designing targeted therapies that inhibit its activity, which could enhance the efficacy of conventional chemotherapeutic agents. The production of recombinant GSTa3 allows for detailed biochemical characterization, enabling researchers to elucidate its role in cellular detoxification pathways and explore its potential as a biomarker or therapeutic target in cancer treatment. As a result, ongoing research aims to reveal new strategies to counteract the adverse effects of GSTa3 overexpression in malignancies, making it a crucial focus in the quest for improved cancer therapeutics.

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