Analytical Data
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Gene name
HCAM
- Application
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Alternative Names
HCAM；Dual specificity calcium/calmodulin-dependent 3'.5'-cyclic nucleotide phosphodiesterase 1A
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P54750
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Expression Region
1-535aa
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AA Sequence
MGSSATEIEELENTTFKYLTGEQTEKMWQRLKGILRCLVKQLERGDVNVVDLKKNIEYAASVLEAVYIDETRRLLDTEDELSDIQTDSVPSEVRDWLASTFTRKMGMTKKKPEEKPKFRSIVHAVQAGIFVERMYRKTYHMVGLAYPAAVIVTLKDVDKWSFDVFALNEASGEHSLKFMIYELFTRYDLINRFKIPVSCLITFAEALEVGYSKYKNPYHNLIHAADVTQTVHYIMLHTGIMHWLTELEILAMVFAAAIHDYEHTGTTNNFHIQTRSDVAILYNDRSVLENHHVSAAYRLMQEEEMNILINLSKDDWRDLRNLVIEMVLSTDMSGHFQQIKNIRNSLQQPEGIDRAKTMSLILHAADISHPAKSWKLHYRWTMALMEEFFLQGDKEAELGLPFSPLCDRKSTMVAQSQIGFIDFIVEPTFSLLTDSTEKIVIPLIEEASKAETSSYVASSSTTIVGLHIADALRRSNTKGSMSDGSYSPDYSLAAVDLKSFKNNLVDIIQQNKERWKELAAQEARTSSQKCEFIHQ
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Molecular Weight
61.2 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The study of HCAM (Hematopoietic Cell Adhesion Molecule), also known as CD44, has garnered significant attention due to its critical role in cell-cell interactions, migration, and signaling in various biological processes. HCAM is predominantly expressed on hematopoietic cells and plays a vital role in lymphocyte homing, proliferation, and activation. Additionally, HCAM is implicated in cancer progression, inflammation, and tissue repair, making it an important target for therapeutic interventions. Research into the recombinant forms of HCAM seeks to elucidate its structure-function relationship, enhance our understanding of its biological roles, and develop potential applications in regenerative medicine and cancer therapy. By engineering HCAM, scientists aim to investigate its interactions with glycosaminoglycans and other ligands that are pivotal for cellular adhesion and communication. Furthermore, the recombinant protein can serve as a valuable tool for studying its mechanism of action in various pathophysiological conditions, thereby providing insights that may lead to innovative treatments for diseases characterized by dysregulated cell adhesion and migration.
