Analytical Data
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Gene name
HLA-DRB4
- Application
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Alternative Names
DR 4; DR beta 4 chain; DR4; DRB1 transplantation antigen ; DRB4; DRB4_HUMAN; HLA class II histocompatibility antigen; HLA class II histocompatibility antigen DR beta 4 chain ; HLA-DRB4; Human leucocyte antigen DRB4 ; Leukocyte antigen ; Major histocompatibility complex class II DR beta 4 ; MHC class II antigen DRB4; MHC class II antigen HLA DR beta ; MHC class2 antigen ; MHC HLA DR-beta chain
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P13762
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Expression Region
30-266aa
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AA Sequence
GDTQPRFLEQAKCECRFLNGTERVWNLIRYIYNQEEYARYNSDLGEYQAVTELGRPDAEYWNSQKDLLERRRAEVDTYCRYNYGVVESFTVQRRVQPKVTVYPSKTQPLQHHNLLVCSVNGFYPGSIEVRWFRNGQEEKAGVVSTGLIQNGDWTFQTLVMLETVPRSGEVYTCQVEHPSMMSPLTVQWSARSESAQSKMLSGVGGFVLGLLFLGTGLFIYFRNQKGHSGLQPTGLLS
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Molecular Weight
51.81 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
HLA-DRB4, a gene encoding a class II major histocompatibility complex (MHC) molecule, plays a crucial role in the immune system by presenting antigens to CD4+ T cells, thereby initiating immune responses. Variants of the HLA-DRB4 gene have been implicated in susceptibility to various autoimmune diseases, such as rheumatoid arthritis and systemic lupus erythematosus, highlighting its significance in both disease pathogenesis and immunogenetics. Research has focused on the recombinant expression of HLA-DRB4 proteins to enable detailed functional studies and the development of diagnostic tools and therapeutic strategies. By producing recombinant HLA-DRB4, scientists aim to characterize its antigen-presenting capabilities, understand how specific alleles interact with different peptides, and evaluate its role in T cell activation and tolerance. These studies contribute to a deeper understanding of the complexities of immune responses and seek to unveil potential targets for intervention in autoimmune disorders. The recombinant protein can also facilitate the development of vaccines and enable the study of T cell epitopes, paving the way for advancements in personalized medicine and immunotherapy. Thus, the investigation of HLA-DRB4 recombinant proteins is a vital area of research that holds promise for enhancing our understanding of immune regulation and disease mechanisms.
