Cat: PA2000-67DB

Recombinant Human CK9 Protein,His

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Analytical Data

  • Gene name

    CK9

  • Application

    SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

  • Alternative Names

    CK9;C11orf15;Transmembrane Protein 9B

  • Species

    Human

  • Source

    E. coli

  • Tag

    His tag N-Terminus

  • Purity

    Greater than 90% as determined by SDS-PAGE.

  • Uniprot

    Q9NQ34

  • Expression Region

    1-198aa

  • AA Sequence

    MATLWGGLLRLGSLLSLSCLALSVLLLAQLSDAAKNFEDVRCKCICPPYKENSGHIYNKNISQKDCDCLHVVEPMPVRGPDVEAYCLRCECKYEERSSVTIKVTIIIYLSILGLLLLYMVYLTLVEPILKRRLFGHAQLIQSDDDIGDHQPFANAHDVLARSRSRANVLNKVEYAQQRWKLQVQEQRKSVFDRHVVLS

  • Molecular Weight

    22.5 kDa

  • Endotoxin

    < 1.0 EU per μg protein as determined by the LAL method.

  • Form

    Freeze-dried powder

  • Buffer formulation

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • Reconstitution

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • Customization

    Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

  • Stability Test

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • Storage & Shelf Life

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • Shipping

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

Quality inspection process

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Protein Description

The study of CK9 recombinant protein is rooted in the broader field of molecular biology and protein engineering, focusing on the significance of cytokeratins in cellular structure and function. Cytokeratin 9, specifically, is a type of intermediate filament protein predominantly expressed in the epithelial tissues of the skin and various organ systems. Its role as a structural component contributes to mechanical resilience and cellular integrity, making it crucial for maintaining tissue architecture. Research into CK9 is particularly important in understanding skin disorders, as mutations or malfunctions of cytokeratins can lead to various dermatological conditions, including palmoplantar keratoderma and epithelial tumors. By utilizing recombinant DNA technology, scientists aim to produce CK9 in a controlled manner, allowing for detailed studies of its structure-function relationships. This has implications not only for basic science but also for potential therapeutic applications, such as developing biomaterials for wound healing or targeted treatments for skin-related diseases. Overall, research on CK9 recombinant protein offers valuable insights into epithelial biology, disease mechanisms, and innovative therapeutic strategies.

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