Analytical Data
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基因名
GFPT1
- Application
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别名
GFPT1；GFAT；GFPT；;Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 1
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q06210-2
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表达区间
1-681aa
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氨基酸序列
MCGIFAYLNYHVPRTRREILETLIKGLQRLEYRGYDSAGVGFDGGNDKDW EANACKIQLIKKKGKVKALDEEVHKQQDMDLDIEFDVHLGIAHTRWATHG EPSPVNSHPQRSDKNNEFIVIHNGIITNYKDLKKFLESKGYDFESETDTE TIAKLVKYMYDNRESQDTSFTTLVERVIQQLEGAFALVFKSVHFPGQAVG TRRGSPLLIGVRSEHKLSTDHIPILYRTGKDKKGSCNLSRVDSTTCLFPV EEKAVEYYFASDASAVIEHTNRVIFLEDDDVAAVVDGRLSIHRIKRTAGD HPGRAVQTLQMELQQIMKGNFSSFMQKEIFEQPESVVNTMRGRVNFDDYT VNLGGLKDHIKEIQRCRRLILIACGTSYHAGVATRQVLEELTELPVMVEL ASDFLDRNTPVFRDDVCFFLSQSGETADTLMGLRYCKERGALTVGITNTV GSSISRETDCGVHINAGPEIGVASTKAYTSQFVSLVMFALMMCDDRISMQ ERRKEIMLGLKRLPDLIKEVLSMDDEIQKLATELYHQKSVLIMGRGYHYA TCLEGALKIKEITYMHSEGILAGELKHGPLALVDKLMPVIMIIMRDHTYA KCQNALQQVVARQGRPVVICDKEDTETIKNTKRTIKVPHSVDCLQGILSV IPLQLLAFHLAVLRGYDVDFPRNLAKSVTVE
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分子量
103 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
GFPT1, or glutamine-fructose-6-phosphate transaminase 1, serves as a critical regulatory enzyme in the hexosamine biosynthetic pathway, which plays a significant role in cellular metabolism and signaling. By catalyzing the conversion of fructose-6-phosphate and glutamine into glucosamine-6-phosphate, GFPT1 contributes to the synthesis of UDP-N-acetylglucosamine, a key substrate for glycosylation processes that affect protein functionality, cell signaling, and structural roles in various physiological systems. Dysregulation of GFPT1 has been linked to several metabolic disorders, including obesity, insulin resistance, diabetes, and certain types of cancer, highlighting its potential as a therapeutic target. Research into recombinant GFPT1 has advanced our understanding of its enzymatic properties, structural characteristics, and how mutations can affect its function. Studies often involve the production of GFPT1 as a recombinant protein, which enables detailed biochemical assays and structural analyses, helping to elucidate its role in metabolic regulation and cell signaling pathways. The ongoing research on GFPT1 aims not only to unravel its basic biological functions but also to explore its implications in disease mechanisms, paving the way for innovative therapeutic strategies in metabolic health and related disorders.
