Analytical Data
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Gene name
CHMP7
- Application
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Alternative Names
CHMP7；Charged multivesicular body Protein 7
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q8WUX9
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Expression Region
1-453aa
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AA Sequence
MWSPEREAEAPAGGDPAGLLPPEWEEDEERMSFLFSAFKRSREVNSTDWDSKMGFWAPLVLSHSRRQGVVRLRLRDLQEAFQRKGSVPLGLATVLQDLLRRGELQRESDFMASVDSSWISWGVGVFLLKPLKWTLSNMLGDNKVPAEEVLVAVELLKEKAEEVYRLYQNSPLSSHPVVALSELSTLCANSCPDERTFYLVLLQLQKEKRVTVLEQNGEKIVKFARGPRAKVSPVNDVDVGVYQLMQSEQLLSRKVESLSQEAERCKEEARRACRAGKKQLALRSLKAKQRTEKRIEALHAKLDTVQGILDRIYASQTDQMVFNAYQAGVGALKLSMKDVTVEKAESLVDQIQELCDTQDEVSQTLAGGVTNGLDFDSEELEKELDILLQDTTKEPLDLPDNPRNRHFTNSVPNPRISDAELEAELEKLSLSEGGLVPSSKSPKRQLEPTLKPL
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Molecular Weight
56.9 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CHMP7 (charged multivesicular body protein 7) is an essential component of the endosomal sorting complex required for transport (ESCRT) pathway, which plays a crucial role in various cellular processes, including vesicle formation, membrane remodeling, and the degradation of membrane proteins. Research has increasingly focused on CHMP7 due to its involvement in cellular responses to stress, regulation of protein turnover, and potential implications in various diseases, including cancer and neurodegenerative disorders. The functional significance of CHMP7 is highlighted by its role in facilitating the formation of multi-vesicular bodies (MVBs) and its involvement in autophagy and cytokinesis. Recent studies have shown that CHMP7 acts as a coordinator for the ESCRT machinery, influencing the efficiency of cellular processes under different physiological conditions. Consequently, the recombinant expression of CHMP7 in various model systems has become a pivotal area of study, providing insight into its structural and functional characteristics. Understanding the biophysical properties and interactions of CHMP7 could unveil new therapeutic targets for conditions characterized by abnormal endosomal trafficking or impaired autophagy. As such, ongoing research into CHMP7 recombinant protein not only deepens our understanding of cellular mechanisms but also paves the way for novel strategies in the treatment of diseases linked to the dysregulation of the ESCRT pathway.
