Analytical Data
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基因名
HERC3
- Application
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别名
HERC3; KIAA0032Probable E3 ubiquitin-protein ligase HERC3; EC 2.3.2.26; HECT domain and RCC1-like domain-containing protein 3; HECT-type E3 ubiquitin transferase HERC3
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q8IXX3
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表达区间
232-358aa
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氨基酸序列
SPCHVKLLRTQKVVYISCGEEHTAVLTKSGGVFTFGAGSCGQLGHDSMNDEVNPRRVLELMGSEVTQIACGRQHTLAFVPSSGLIYAFGCGARGQLGTGHTCNVKCPSPVKGYWAAHSGQLSARADR
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分子量
117 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
HERC3 (HECT and RLD domain containing E3 ubiquitin protein ligase 3) is a member of the HECT E3 ubiquitin ligase family, which plays a critical role in post-translational modification processes by ubiquitination, impacting protein stability and cellular functions. The study of HERC3 has garnered attention due to its involvement in various physiological and pathological processes, including immune response regulation, cell proliferation, and apoptosis. Research indicates that aberrant expression or dysfunction of HERC3 may contribute to diverse diseases, such as cancer and neurodegenerative disorders. Understanding HERC3's mechanisms can reveal important insights into these pathologies and highlight potential therapeutic targets. Recent studies utilizing recombinant HERC3 proteins have aimed to elucidate its substrate specificity, interaction networks, and functional roles within the cell, thus paving the way for advancements in targeted therapies. The generation of recombinant HERC3 also facilitates biomechanical assays and high-throughput screening, providing a platform for identifying novel inhibitors or modulators of HERC3 activity. Consequently, ongoing research into HERC3 and its recombinant forms represents a promising frontier in molecular biology, with implications for improving disease outcomes through therapeutic intervention.
