Analytical Data
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Gene name
GCAT
- Application
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Alternative Names
GCAT；KBL;2-amino-3-ketobutyrate coenzyme A ligase. mitochondrial
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O75600
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Expression Region
22-419aa
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AA Sequence
MGSSHHHHHHSSGLVPRGSHMSALAQLRGILEGELEGIRGAGTWKSERVI TSRQGPHIRVDGVSGGILNFCANNYLGLSSHPEVIQAGLQALEEFGAGLS SVRFICGTQSIHKNLEAKIARFHQREDAILYPSCYDANAGLFEALLTPED AVLSDELNHASIIDGIRLCKAHKYRYRHLDMADLEAKLQEAQKHRLRLVA TDGAFSMDGDIAPLQEICCLASRYGALVFMDECHATGFLGPTGRGTDELL GVMDQVTIINSTLGKALGGASGGYTTGPGPLVSLLRQRARPYLFSNSLPP AVVGCASKALDLLMGSNTIVQSMAAKTQRFRSKMEAAGFTISGASHPICP VMLGDARLASRMADDMLKRGIFVIGFSYPVVPKGKARIRVQISAVHSEED IDRCVEAFVEVGRLHGALP
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Molecular Weight
45 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The study of genetically engineered proteins, particularly the GCAT (Glutamate-Cysteine Ligase Catalytic Subunit), has garnered significant interest due to its critical role in cellular functions and disease mechanisms. GCAT is an enzyme involved in the synthesis of glutathione, a vital antioxidant that protects cells from oxidative stress and plays a crucial role in detoxification processes. Research has shown that dysregulation of GCAT can contribute to various health issues, including neurodegenerative diseases, cancer, and metabolic disorders. Scientists are exploring recombinant protein technology to produce GCAT for various applications, including therapeutic interventions and as a tool for studying cellular redox states. By leveraging recombinant DNA technology, researchers aim to understand the enzyme's structure-function relationships, its interactions with other biomolecules, and how it can be manipulated for potential clinical benefits. This research not only enhances the understanding of cellular redox biology but also opens avenues for developing novel strategies to combat diseases linked to oxidative stress, making GCAT a promising target for future biomedical research.
